CsIPT1b and CsUGT85A2 delay female corolla opening in cucumber by regulating the content of cytokinins  

作　　者：Xiaoli Li Haonan Cui Xiaofei Song Chengzhen Sun Zhuo Ding Xueyun Zhu Xiaofeng Liu Liying Yan 

机构地区：[1]Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,College of Horticulture Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004,China [2]College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China [3]Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province,Qingdao,Shandong 266109,China

出　　处：《Horticultural Plant Journal》2023年第4期754-762,共9页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.31772327);S&T Program of Hebei(Grant No.21326309D).

摘　　要：Corolla opening is essential for the propagation of unisexual flowering plants,meanwhile commodity fruits with opening corolla have higher commodity value in cucumber fresh food market.Until now,there is little research on the mechanism of corolla opening in cucumber.In a previous paper,we identified a unique cucumber(Cucumis sativus)line(‘6457’)that possesses super ovaries and shows delayed corolla opening when nutrient supplies are abundant.We also previously showed that the expression of CsIPT1b(isopentenyl transferase)and CsUGT85A2(cytokinin glycosyltransferase)is correlated with the delayed opening of the female corolla.Here,we investigated the mechanism of delayed female corolla opening in cucumber by conducting transgenic experiments,hormone assays,and yeast one-hybrid assays.CsIPT1b and CsUGT85A2 positively and negatively regulated delayed female corolla opening,respectively.In CsIPT1b-overexpressing plants and CsUGT85A2 knockout plants,the content of the trans-zeatin riboside,trans-zeatin,and N6-isopentenyladenosine was increased.Overall,our findings indicate that CsIPT1b and CsUGT85A2 were important regulators of the timing of female corolla opening.These genes could thus be targets to cucumber molecular breeding of fruit with flowers remaining on the tip.

关 键 词：Cucumis sativus CsIPT1b CsUGT85A2 Corolla opening 

分 类 号：S642.2[农业科学—蔬菜学]