超高效液相色谱-串联质谱法同时测定草莓中 咯菌腈和异菌脲残留量  

Determination of Fludioxonil and Iprodione Residues in Strawberry by UPLC-MS/M

作　　者：赵圣英 高祥文 于建垒[2] 刘同金[2] 梁慧 房锋[2] 李瑞娟[2] ZHAO Shengying;GAO Xiangwen;YU Jianlei;LIU Tongjin;LIANG Hui;FANG Feng;LI Ruijuan(Shandong Shibang Agricultural Chemitry Co.,LTD,Jinan250100,China;Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan250100,China)

机构地区：[1]山东仕邦农化有限公司,济南250100 [2]山东省农业科学院植物保护研究所,济南250100

出　　处：《现代农药》2023年第4期69-72,共4页MODERN AGROCHEMICALS

基　　金：山东省农业科学院农业科技创新工程(CXGC2023D01);农业农村部农药登记残留试验项目(PR2022-005)。

摘　　要：为了建立一种同时检测草莓中咯菌腈和异菌脲残留量的分析方法,本研究采用乙腈提取草莓样品中的咯菌腈和异菌脲,GCB、PSA净化,超高效液相色谱-串联质谱检测,外标法定量。结果表明,咯菌腈和异菌脲的质量浓度与峰面积间在0.01~1 mg/L范围内呈良好线性关系,咯菌腈在0.01~3.0 mg/kg添加水平下,平均回收率为83%~105%,相对标准偏差为2.0%~12.1%;异菌脲在0.01~10 mg/kg添加水平下,平均回收率为93%~101%,相对标准偏差为4.9%~9.4%,咯菌腈和异菌脲的最低检出浓度均为0.01 mg/kg。该方法简单、灵敏度高、重现性好,适用于草莓样品中同时测定咯菌腈和异菌脲的残留量。This study aims to develop a new method for residue analysis of fludioxonil and iprodione in strawberry by UPLC-MS/MS.Fludioxonil and iprodione residues in strawberry were extracted with acetonitrile,purified by GCB and PSA,and determined by UPLC-MS/MS.The quantitative analysis was carried out by external standard method.The results showed that fludioxonil and iprodione showed a good linear relationship at the mass concentration range of 0.01-1 mg/L.At the addition level of 0.01-3.0 mg/kg,the average recoveries of fludioxonil in strawberry were 83%-105%,the relative standard deviations were 2.0%-12.1%.At the addition level of 0.01-10 mg/kg,the average recoveries of iprodione in strawberry were 93%-101%,the relative standard deviations were 4.9%-9.4%.The detection limit of fludioxonil and iprodione were 0.01 mg/kg.The method is simple and reliable,sensitive and reproducible,which can be applied for determining fludioxonil and iprodione in strawberry samples.

关 键 词：咯菌腈 异菌脲 草莓 超高效液相色谱-串联质谱法 残留 

分 类 号：TQ450.2[化学工程—农药化工]