三阴性乳腺癌患者ASH2L、HOXA2表达及其与淋巴转移的关系  

Expressions of ASH2L and HOXA2 in triple-negative breast cancer patients and their relationship with lymph node metastasis

作　　者：陈琛[1] 段奇 陆佳团 翟晓建[1] 王征[1] 张浩[1] 郭满[2] CHEN Chen;DUAN Qi;LU Jiatuan;ZHAI Xiaojian;WANG Zheng;ZHANG Hao;GUO Man(Ward 1,Department of Breast,Nanyang Central Hospital,Nanyang 473000,Henan,China;Department of Thyroid,Nanyang Central Hospital,Nanyang 473000,Henan,China)

机构地区：[1]南阳市中心医院乳腺科一病区,河南南阳473000 [2]南阳市中心医院甲状腺科,河南南阳473000

出　　处：《检验医学》2023年第6期574-578,共5页Laboratory Medicine

基　　金：河南省科技发展计划项目(202102310096)。

摘　　要：目的探讨三阴性乳腺癌(TNBC)患者ASH2样蛋白(ASH2L)和同源异型盒A2(HOXA2)的表达及其与淋巴转移的关系。方法选取2016年6月—2020年6月南阳市中心医院TNBC患者80例,收集其癌组织样本;另收集60例配对癌旁组织(距肿瘤边缘≥5 cm)样本作为对照。收集所有患者的临床病理资料,并检测ASH2L、HOXA2 mRNA和蛋白的表达。采用受试者工作特征(ROC)曲线评价ASH2L mRNA、HOXA2 mRNA判断TNBC患者淋巴转移的效能。结果癌组织中ASH2L mRNA、HOXA2 mRNA相对表达量和蛋白阳性率均显著高于癌旁组织(P<0.05)。有淋巴转移、TNM分期Ⅲ~Ⅳ期的TNBC患者癌组织ASH2L蛋白、HOXA2蛋白阳性率分别显著高于无淋巴转移和TNM分期Ⅰ~Ⅱ期的患者(P<0.05)。不同年龄、肿瘤大小、绝经状态的TNBC患者之间癌组织ASH2L蛋白、HOXA2蛋白阳性率差异无统计学意义(P>0.05)。有淋巴转移的TNBC患者癌组织中ASH2L mRNA、HOXA2 mRNA相对表达量显著高于无淋巴转移的患者(P<0.05)。ASH2L mRNA、HOXA2 mRNA单项检测和联合检测诊断TNBC患者淋巴转移的曲线下面积(AUC)分别为0.706、0.791、0.895。结论TNBC患者癌组织中ASH2L、HOXA2 mRNA和蛋白均呈高表达,且与TNM分期和淋巴转移有关。Objective To investigate the expressions of ASH2-like protein(ASH2L)and homeobox A2(HOXA2)in triple-negative breast cancer(TNBC)patients and their relationship with lymph node metastasis.Methods A total of 80 TNBC patients from Nanyang Central Hospital from June 2016 to June 2020 were enrolled,and their cancer tissue samples were collected.Totally,60 paired cancer adjacent tissue samples(≥5 cm from the edges of the tumors)were collected as controls.The clinical and pathological data were collected,and the expressions of ASH2L,HOXA2 mRNA and proteins were determined.The efficacy of ASH2L mRNA and HOXA2 mRNA in determining lymph node metastasis in TNBC patients was evaluated by receiver operating characteristic(ROC)curve.Results The relative expression levels of ASH2L mRNA,HOXA2 mRNA and protein positive rate were higher in cancer tissues than those in adjacent tissues(P<0.05).The positive rates of ASH2L protein and HOXA2 protein in cancer tissues of TNBC patients with lymph node metastasis and TNM stageⅢ-Ⅳwere higher than those of TNBC patients without lymph node metastasis and TNM stageⅠ-Ⅱ(P<0.05).There was no statistical significance in the positive rates of ASH2L protein and HOXA2 protein in cancer tissues from TNBC patients of different ages,tumor sizes and menopausal status(P>0.05).The relative expression levels of ASH2L mRNA and HOXA2 mRNA in cancer tissues of TNBC patients with lymph node metastasis were higher than those without lymph node metastasis(P<0.05).The areas under curves(AUC)of ASH2L mRNA and HOXA2 mRNA single determinations and combined determination for diagnosing lymph node metastasis in TNBC patients were 0.706,0.791 and 0.895,respectively.Conclusions The ASH2L and HOXA2 mRNA and proteins are highly expressed in cancer tissues of TNBC patients,and they are related with TNM staging and lymph node metastasis.

关 键 词：ASH2样蛋白 同源异型盒A2 淋巴转移 三阴性乳腺癌 

分 类 号：R446.1[医药卫生—诊断学]