香港牡蛎在综合养殖池塘育肥期间不同组织的碳、氮稳定同位素周转  

Stable carbon and nitrogen isotope turnover of different tissues of the Hongkong oyster at the fattening stage in a polyculture pond

作　　者：周晖[1] 汤保贵[1] 伍栩民 彭梓峰 钟培贵 于鸽 孔繁森 ZHOU Hui;TANG Baogui;WU Xumin;PENG Zifeng;ZHONG Peigui;YU Ge;KONG Fansen(Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China;Haiste(Zhanjiang)Aquatic Technology Co.,Ltd.,Zhanjiang 524099,China;Guangdong Evergreen Breeding Technology Service Co.,Ltd.,Zhanjiang 524094,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]湛江海思特水产科技有限公司,广东湛江524099 [3]湛江恒兴养殖技术服务有限公司,广东湛江524094

出　　处：《热带海洋学报》2023年第4期125-132,共8页Journal of Tropical Oceanography

基　　金：国家海洋局“十三五”海洋经济创新发展示范市项目(XM-202012-03B2)。

摘　　要：本研究采集了在鱼虾混养池塘育肥的香港牡蛎样品,监测牡蛎体质量、软体组织质量、出肉率,以及闭壳肌、外套膜和鳃的δ^(13)C和δ^(15)N值随育肥时间的变化,计算了牡蛎3种软体组织的代谢率常数m和碳、氮稳定同位素周转时间,为开展综合养殖池塘生态系统的食物网研究提供基础数据。结果显示:经过44d的育肥,虽然牡蛎体质量无显著增加(P>0.05),但软体组织质量和出肉率显著上升(P<0.05)。育肥期间牡蛎外套膜、闭壳肌和鳃的生长与整个软体组织的生长基本同步。牡蛎各组织的δ^(13)C和δ^(15)N值在育肥16d后就显著减小(P<0.05)。比较同一天采集的组织,牡蛎闭壳肌的δ^(13)C和δ^(15)N值都显著大于外套膜和鳃(P<0.05),而外套膜和鳃的δ^(13)C和δ^(15)N值则无显著差异(P>0.05)。闭壳肌和外套膜的代谢周转常数m为0,其碳、氮稳定同位素周转完全由生长决定,因此闭壳肌和外套膜碳、氮稳定同位素的周转时间相同,t50和t95分别为40.77d和176.22d。鳃的碳、氮稳定同位素代谢周转常数m分别为1.09%·d^(-1)和1.76%·d^(-1),说明鳃的碳、氮稳定同位素周转还受到代谢的影响;鳃氮稳定同位素周转的t50和t95分别为20.02d和86.53d,而碳稳定同位素周转的t50和t95分别为24.85d和107.42d。在本研究的育肥期间,香港牡蛎闭壳肌、外套膜和鳃的δ13C和δ15N值具有鲜明特点,并且随育肥时间延长呈现出迅速且有规律的变化,表明香港牡蛎能够反映池塘的碳、氮稳定同位素基本特征,适合作为中、长时间尺度的基线生物用于综合养殖池塘生态系统的食物网研究。In order to provide basic data for the study of food webs in the polyculture pond ecosystem,the Hongkong oyster samples were collected during fattening stage in a fish-shrimp polyculture pond,and the oyster body mass,soft tissue mass,meat yield,and changes inδ^(13)C andδ^(15)N values of adductor muscle,mantle and ctenidia during the fattening stage were monitored;the metabolic rate constant m and stable carbon and nitrogen isotope turnover time of three soft tissues were calculated.The results showed that after 44 days of fattening,although the body mass of oysters did not increase significantly(P>0.05),the soft body tissue mass and meat yield increased significantly(P<0.05).The growth of mantle,adductor muscle and ctenidia were basically synchronized with the growth of whole soft tissue.Theδ^(13)C and δ^(15)N values of oyster tissues decreased significantly(P<0.05)after 16 days of fattening(P<0.05).Comparing theδ^(13)C andδ^(15)N values of the three tissues collected on the same day,the values of adductor muscle were significantly larger than those of mantle and ctenidia(P<0.05),while there was no significant difference between values of mantle and ctenidia(P>0.05).The metabolic turnover constant m of adductor muscle and mantle was zero,and their stable carbon and nitrogen isotope turnover were completely determined by growth;therefore the turnover time of stable carbon and nitrogen isotope turnover in adductor muscle and mantle were the same,t50 and t95 were 40.77d and 176.22d,respectively.The stable carbon and nitrogen isotope metabolic turnover constant m of ctenidia were 1.09%·d^(−1) and 1.76%·d^(−1),respectively,indicating that the stable carbon and nitrogen isotope turnover of ctenidia were also affected by metabolism.The t50 and t95 of ctenidia stable nitrogen isotope turnover were 20.02d and 86.53d,respectively,while the t50 and t95 of stable carbon isotope turnover were 24.85d and 107.42d,respectively.During the fattening stage of this study,theδ^(13)C andδ^(15)N values of the adductor mus

关 键 词：香港牡蛎 育肥 碳、氮稳定同位素 周转 同位素基线 

分 类 号：P735.2[天文地球—海洋生物学]