除草剂乙草胺胁迫下两种软珊瑚生理响应  

Physiological responses of two soft corals to herbicide acetochlor stress

作　　者：郁强 董娴娴 李长青 张欣 赵国理 CHIN Yaoxian 王沛政 YU Qiang;DONG Xianxian;LI Changqing;ZHANG Xin;ZHAO Guoli;CHIN Yaoxian;WANG Peizheng(Hainan Provincial Key Laboratory of Marine Ecological Environment Processes and Carbon Sequestration,Sanya 572022,China;Yazhou Bay Innovation Research Institute,Hainan Institute of Tropical Oceanography,Sanya 572022,China;Hainan Sanya National Coral Reef Nature Reserve Management Office,Sanya 572000,China)

机构地区：[1]海南省近岸海洋生态环境过程与碳汇重点实验室,海南三亚572022 [2]海南热带海洋学院崖州湾创新研究院,海南三亚572022 [3]海南三亚国家级珊瑚礁自然保护区管理处,海南三亚572000

出　　处：《热带海洋学报》2023年第4期133-145,共13页Journal of Tropical Oceanography

基　　金：海南省自然科学基金(321MS0821)。

摘　　要：文章以乙草胺(acetochlor,ACT)为环境胁迫因子,利用不同浓度的乙草胺对肉质软珊瑚(Sarcophyton sp.)和短指软珊瑚(Sinularia sp.)进行长期和短期胁迫,以研究胁迫下的珊瑚生理指标变化情况。研究结果显示,肉质软珊瑚和短指软珊瑚受到不同浓度乙草胺胁迫后,珊瑚的叶绿素a和虫黄藻含量呈现先升高后降低的趋势,超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性持续升高然后降低并保持低活性水平,碱性磷酸酶(alkaline phosphatase,AKP)活性先升高后逐渐降低,谷胱甘肽巯基转移酶(glutathione s-transferase,GST)活性升高并保持在较高水平。胁迫后肉质软珊瑚的共生虫黄藻多样性变低,而短指软珊瑚的共生虫黄藻则是丰富度变低。丰度高的虫黄藻都属于系群C,而短指软珊瑚G系群丰度升高。胁迫后软珊瑚Cyanobacteria(蓝细菌)丰度逐渐增加,Firmicutes(厚壁菌门)丰度逐渐减少。以上结果表明,肉质软珊瑚(Sarcophyton sp.)和短指软珊瑚(Sinularia sp.)在受到乙草胺胁迫后,其珊瑚生理指标产生了较大变化。In this paper,using acetochlor(ACT)as the stress environment,the long-term and short-term stress of Sarcophyton sp.and Sinularia sp.with different concentrations of acetochlor were used to study the stress changes in coral physiological indicators.Studies have shown that the chlorophyll a and zooxanthellae contents of succulent soft corals and brachydactyly soft corals increased first and then decreased after different concentrations of acetochlor stress.Superoxide dismutase(SOD)and Catalase(CAT)activity will continue to increase and then decrease and maintain a low level,Alkaline Phosphatase(AKP)activity will first increase and then gradually decrease,Glutathione S-Transferase(GST)activity increased and maintained at a high level.The symbiotic zooxanthellae diversity of succulent soft corals became lower,while the abundance of symbiotic zooxanthellae of Brachydactyly soft corals became lower.The zooxanthellae with high abundance all belonged to group C,while the abundance of group G of Brachydactyly was increased.The abundance of soft coral Cyanobacteria gradually increased and the abundance of Firmicutes gradually decreased after stress.The above studies showed that acetochlor had great changes in coral physiological indicators after the stress of Sarcophyton sp.and Sinularia sp..

关 键 词：软珊瑚 乙草胺 虫黄藻 生理指标 响应机制 

分 类 号：P735.542[天文地球—海洋生物学]