太乙膏对大鼠深Ⅱ度烫伤创面肉芽组织中表皮生长因子水平的影响  

Effect of Taiyi Ointment on the Level of Epidermal Growth Factor in Granulation Tissue of Deep Second Degree Scald Wounds in Rats

作　　者：张博 ZHANG Bo(Department of Anorectal II,Qingyang Traditional Chinese Medicine Hospital,Qingyang 745000,China)

机构地区：[1]庆阳市中医医院肛肠二科,甘肃庆阳745000

出　　处：《中西医结合研究》2023年第4期234-238,共5页Research of Integrated Traditional Chinese and Western Medicine

基　　金：甘肃省中医药管理局科研课题(No.GZK-2018-76)。

摘　　要：目的 观察太乙膏对于大鼠深Ⅱ度烫伤创面肉芽组织中表皮生长因子(epidermal growth factor,EGF)、血管内皮生长因子(vascular endothelial growth factor,VEGF)含量的影响,以期探讨太乙膏在促进创面愈合过程中的作用机制。方法 SPF级Wistar大鼠60只,空白对照组6只,剩余54只建立大鼠深Ⅱ度烫伤模型。烫伤模型大鼠按照随机数字表法分为太乙膏组、解毒生肌膏组、模型对照组,每组18只。3组大鼠分别在烫伤部位涂抹太乙膏、解毒生肌膏及0.9%氯化钠注射液。连续治疗3周后,切取大鼠背部创面肉芽组织,采用免疫组化法检测肉芽组织中EGF、VEGF水平。结果 解毒生肌膏组、太乙膏组大鼠烫伤创面肉芽组织中EGF、VEGF水平显著高于模型对照组(P<0.05),且太乙膏组EGF、VEGF水平明显高于解毒生肌膏组(P<0.05)。结论 太乙膏通过某种调控促使创面肉芽组织中EGF、VEGF含量增多,可能是其促进创面愈合的作用机理。Objective To observe the effect of Taiyi ointment on the contents of epidermal growth factor(EGF)and vascular endothelial growth factor(VEGF)in granulation tissue of deep second degree scald wound in rats,so as to explore the mechanism of Taiyi ointment in promoting wound healing.Methods Sixty SPF grade Wistar rats,6 rats in the blank control group,the remaining 54 rats were used to establish deepⅡdegree scald model.Scald model rats were randomly divided into Taiyi ointment group,Jiedu Shengji ointment group,and model control group using a random number table method,with 18 rats in each group.Three groups of rats were coated with Taiyi ointment,Jiedu Shengji ointment,and 0.9%sodium chloride injection on the scald site.After 3 weeks of continuous treatment,granulation tissue from the back wound of rats was harvested and the levels of EGF and VEGF in the granulation tissue were detected using immunohistochemistry.Results The levels of EGF and VEGF in the granulation tissue of scalded wounds in the Jiedu Shengji ointment group and Taiyi ointment group were significantly higher than those in the model control group(P<0.05),and the levels of EGF and VEGF in the Taiyi ointment group were significantly higher than those in the Jiedu Shengji ointment group(P<0.05).Conclusion Taiyi ointment promotes the increase of EGF and VEGF content in wound granulation tissue through certain regulation,which might be its mechanism of promoting wound healing.

关 键 词：太乙膏 深Ⅱ度烫伤 创面肉芽组织 表皮生长因子 血管内皮生长因子 

分 类 号：R644[医药卫生—外科学]