芦笋试管苗茎段组培生根的关键技术初探  

Key techniques of stem segment rooting in tissue culture of Asparagus officinicalL.

作　　者：包艳存 李保华[1] 牟萌[1] 牛晓雪 路远 李霞[1] BAO Yancun;LI Baohua;MU Meng;NIU Xiaoxue;LU Yuan;LI Xia(Asparagus Research Institute,Weifang Academy of Agricultural Sciences,Weifang 261071,Shandong,China)

机构地区：[1]潍坊市农业科学院芦笋研究所,山东潍坊261071

出　　处：《中国瓜菜》2023年第8期99-104,共6页China Cucurbits And Vegetables

基　　金：山东省重点研发计划(农业良种工程)项目(2022LZGC024);潍坊市科技发展计划(2021ZJ1128);潍坊市科技发展计划(2022ZJ1084)。

摘　　要：为实现芦笋组培苗茎尖、茎段同步生根,提高组培快繁效率,在试管苗茎尖生根的基础上,以4个不同的芦笋组培继代增殖试管苗为试验材料,研究了茎段部位、茎段直径大小、不同激素配比对芦笋试管苗茎段生根的影响。结果表明,经过4代以上继代培养的增殖试管苗可通过茎段生根获得完整植株,移栽成活率最高可达85.3%。茎段选取部位以茎尖下1~4节的茎段为宜,对于常规的试管苗,宜选取直径1.5~2.5 mm的茎段;对于特别细的试管苗,宜选取直径1.0~2.0 mm的茎段。在生根培养基中添加适当浓度的Ancymidol能显著提高试管苗的生根率,添加质量浓度以0.10 mg·L^(-1)为宜。In order to realize simultaneous rooting of shoot tip and stem segment in tissue culture of asparagus,and to improve the efficiency of tissue culture and rapid propagation,the four different subculture proliferation plantlets of aspar-agus were used as the experimental materials,the stem segment position,stem diameter and different hormone proportion on rooting was studied in this experiment.The results showed that asparagus subculture proliferation seedlings after four or more generations can be obtained by rooting the stem segments,the survival rate of transplanting can reach 85.3%.The stem segments with 1-4 nodes below the tip should be selected.For conventional asparagus tube seedlings,the stem segments with diameter of 1.5-2.5 mm should be selected.For asparagus tube seedlings with very thin stems,the stem segments with diameter of 1.0-2.0 mm should be selected.Adding the appropriate concentration of Ancymidol to the rooting medium,the availability rooting rate was significantly improved.The appropriate concentration is 0.10 mg∙L^(-1).

关 键 词：芦笋 组培试管苗 茎段 生根 

分 类 号：S644.6[农业科学—蔬菜学]