中国内地地区埃可病毒25型全基因组进化与重组分析  

Whole Genome Evolution and Recombination Analysis of Echovirus 25 in Chinese Mainland

作　　者：王晓艺 李世康[3] 寸建萍[4] 施勇 刘莹莹[6] 卫海燕[7] 张勇[2] 王雯慧 肖金波 宋洋[2] 严冬梅[2] 杨倩[2] 孙强[2] 冀天娇[2] 许文波[1,2] WANG Xiaoyi;LI Shikang;CUN Jianping;SHI Yong;LIU Yingying;WEI Haiyan;ZHANG Yong;WANG Wenhui;XIAO Jinbo;SONG Yang;YAN Dongmei;YANG Qian;SUN Qiang;JI Tianjiao;XU Wenbo(Medical School,Anhui University of Science and Technology,Huainan 232001,China;World Health Organization Western Pacific Region Polio Reference Laboratory,National Health and Wellness Commission Key Laboratory of Medical Virology and Viral Diseases,Chinese Center for Disease Control and Prevention Institute of Viral Disease Prevention and Control,Beijing 102206,China;Hunan Center for Disease Control and Prevention,Changsha 410005,China;Yunnan Center for Disease Control and Prevention,Kunming 650100,China;Jiangxi Center for Disease Control and Prevention,Nanchang 330006,China;Hebei Center for Disease Control and Prevention,Shijiazhuang 050000,China;Henan Center for Disease Control and Prevention,Zhengzhou 450000,China;Shandong First Medical University(Shandong Academy of Medical Sciences)School of Public Health and Health Management,Jinan 250117,China)

机构地区：[1]安徽理工大学医学院,淮南232001 [2]世界卫生组织西太平洋区脊髓灰质炎参比实验室,国家卫生和健康委员会医学病毒学和病毒病重点实验室,中国疾病预防控制中心病毒病预防控制所,北京102206 [3]湖南省疾病预防控制中心,长沙410005 [4]云南省疾病预防控制中心,昆明650100 [5]江西省疾病控制预防中心,南昌330006 [6]河北省疾病预防控制中心,石家庄050000 [7]河南省疾病预防控制中心,郑州450000 [8]山东第一医科大学(山东省医学科学院)公共卫生与健康管理学院,济南250117

出　　处：《病毒学报》2023年第4期1008-1016,共9页Chinese Journal of Virology

基　　金：国家重点研发计划“病原学与防疫技术体系研究”重点专项项目“重要急性传染病医防协同病原基因组学监测预警和溯源技术体系研究”(项目号:2022YFC2305300),题目:急性肠道传染病病原体基因组和关键甄别位点参比数据库的建立。

摘　　要：埃可病毒25型(Echovirus 25,E25)属于肠道病毒B组(Enterovirus B,EV⁃B),其在临床上可引起包括手足口病(Hand,foot,and mouth disease,HFMD),无菌性脑膜炎(Aseptic meningitis,AM),急性弛缓性麻痹(Acute flaccid paralysis,AFP)等在内的多种疾病。本文从全国HFMD监测网络选取了2009⁃2018年的18株E25毒株进行全基因组测序,将所得全长序列及GenBank上目前已发表的E25全长序列,以及EV⁃B组其它原型株全长序列分别构建全基因组和各编码区的亲缘进化树,并依据不同进化分支选取具有代表性的两株E25毒株进行重组分析。结果显示,中国内地地区E25在P1区具有更高的亲缘关系,而P2和P3区建树中各出现了两个分支,且在每个分支上,与多个EV⁃B组的原型株具有较高的核苷酸同源性,提示E25可能存在多种重组模式。此外所选的两株毒株均与EV⁃B的其它血清型流行株在P2及P3不同区域发生重组,且重组的模式不相同,因此中国内地地区的E25至少存在两种重组模式,并且致病力可能与基因的重组有关。通过本研究的分析,进一步的揭示中国内地地区E25的重组特征,对其进化变异及后续的预防与治疗具有重要意义。Echovirus 25(E25)belongs to Enterovirus B(EV⁃B),which can cause many diseases including hand,foot,and mouth disease(HFMD),aseptic meningitis(AM),acute flaccid paralysis(AFP)and so on.In this paper,18 strains of E25 virus from 2009 to 2018 were selected from the National Hand⁃foot⁃mouth Disease Detection Network for whole⁃genome sequencing.The full⁃length sequence obtained and the full⁃length sequence of E25 currently published on GenBank,as well as the full⁃length sequence of other prototype strains of EV⁃B group,were selected to construct phylogenetic trees of the whole genome and each coding region,and two representative strains of E25 virus were selected according to different evolutionary branches for recombination analysis.The results showed that E25 in Chinese Mainland was relatively conservative in P1 region,but two branches appeared in P2 and P3 regions,and showed higher nucleotide similarity with different types of prototype strains,suggesting that E25 may have multiple recombination patterns.In addition,two selected strains were recombined with other serotype epidemic strains of EV⁃B group in different regions of P2 and P3,and the recombination mode was different.Therefore,E25 in Chinese Mainland has at least two recombination modes,and its pathogenicity may be related to gene recombination.Through the analysis of this study,we can further reveal the recombination characteristics of E25 in Chinese Mainland,which is of great significance for its evolutionary variation and subsequent prevention and treatment.

关 键 词：埃可病毒25型 全基因组 基因重组 

分 类 号：R373.2[医药卫生—病原生物学]