猪轮状病毒感染小鼠肠道组织的转录组分析  

Transcriptome Analysis of Intestinal Tissue Infected with Porcine Rotavirus in Mice

作　　者：李颖 李树东[2] 秦达 曲艺 程荣叶 侯喜林[2] 余丽芸[1] LI Ying;LI Shudong;QIN Da;QU Yi;CHENG Rongye;HOU Xilin;YU Liyun(Heilongjiang Bayi Agricultural University,College of Life Science,Daqing 163319,China;Heilongjiang Bayi Agricultural University,College of Animal Science and Technology,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]黑龙江八一农垦大学动物科学技术学院,大庆163319

出　　处：《病毒学报》2023年第4期1072-1083,共12页Chinese Journal of Virology

基　　金：“三纵”科研团队(自然)项目(项目号:TDJH201904),题目:动物肠道菌群与免疫学研究创新团队;黑龙江八一农垦大学研究生创新科研项目(项目号:YJSCX2021-Y101)题目:类干酪乳杆菌对轮状病毒损伤的上皮细胞的修复作用的研究

摘　　要：猪轮状病毒(Porcine rotavirus,PoRV)是在世界范围内与严重腹泻疾病相关的主要肠道病原体,是新生仔猪肠炎和腹泻致死的重要原因之一。为了深入了解猪轮状病毒感染肠道后其致病机制以及引起宿主的抗病毒和修复机制,我们分别饲喂培养基和猪轮状病毒病毒液5d后,采取5 d、10 d、15 d、20 d、25 d小鼠空肠组织进行高通量转录组测序分析。利用基因本体论(Gene Ontology,GO)数据库功能富集分析、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析差异表达基因(Differentially Expressed Gene,DEGs),选取部分差异表达基因,进行qRT⁃PCR验证。结果显示,与对照组相比,5个时间段上调DEGs有849个,下调DEGs有824个。对DEGs进行5个功能富集,有共同差异基因15个。这些差异表达基因广泛参与脂质合成与代谢、免疫、细胞增殖、细胞凋亡等活动,主要注释到PPAR、NOD⁃like、IL⁃17等信号通路中。qRT⁃PCR验证上皮细胞增殖相关基因PBLD、CCL24、Nr1d1、Fst表达趋势与RNA⁃seq结果趋势一致。此分析提示PoRV感染小鼠肠上皮细胞后可能通过细胞增殖相关基因的高表达或其信号通路的激活进行受损的肠上皮的修复。Porcine rotavirus(PoRV)is the leading intestinal pathogen associated with severe diarrhea in the world,and is one of the important causes of enteritis and diarrhea in newborn piglets.In order to further understand the pathogenic mechanism of PoRV infection and the antiviral and repair mechanism of the host,mouse jejunum tissue was analyzed by high throughput transcriptome sequencing after feeding the mice with culture medium or porcine rotavirus solution for 5 days respectively.Functional enrichment analysis of GO database and KEGG pathway were used to analyze DEGs.Some differentially expressed genes were selected and verified by qRT⁃PCR.The results showed that compared with the control group,849 DEGs were up⁃regulated and 824 DEGs were down⁃regulated in 5 time periods.DEGs enriched in 5 functions and 15 common differential genes were found.These differentially expressed genes are involved in lipid synthesis and metabolism,immunity,cell proliferation,apoptosis and other activities,mainly annotated to PPAR,NOD⁃like,IL⁃17 and other signal pathways.The expression trend of epithelial cell proliferation related genes PBLD,CCL24,Nr1d1 and Fst verified by qRT⁃PCR was consistent with that of RNA⁃seq.The analysis suggests that the damaged mouse intestinal epithelial cells may be repaired through high expression of cell proliferation⁃related genes or activation of their signal pathway after PoRV infection.

关 键 词：猪轮状病毒 差异表达基因 转录组测序分析 小鼠肠道组织 

分 类 号：R512.5[医药卫生—内科学]