黑曲霉产酸调控及其浸出碳硅泥岩型铀矿石  

Regulation of Acid Production by Aspergillus Niger and Its LeachingPerformance for Carbon-Silica Mudstone Type Uranium Ore

作　　者：徐克灵 孙静 李广悦 XU Keling;SUN Jing;LI Guangyue(School of Resources,Environment and Safety Engineering,University of South China,Hengyang 421001,China)

机构地区：[1]南华大学资源环境与安全工程学院,湖南衡阳421001

出　　处：《有色金属工程》2023年第8期62-67,77,共7页Nonferrous Metals Engineering

基　　金：国家自然科学基金资助项目(51674146,U20A20267)。

摘　　要：采用黑曲霉发酵液浸出铀矿石,发酵液中的有机酸浓度直接影响铀的浸出。为了提高黑曲霉发酵液对碳硅泥岩型铀矿的浸出效果,通过设计正交试验对黑曲霉的产酸条件进行优化,研究了预培养时间、微粒子浓度、接种比例、发酵培养时间对黑曲霉代谢产酸的调控作用,分析了发酵液中有机酸含量与铀浸出率的关系。结果表明,在预培养时间15 h、微粒子浓度5 g/L、黑曲霉接种比例4%、发酵培养时间96 h的最优试验条件下,黑曲霉发酵液中的总酸浓度为6 880 mg/L,利用该条件下的发酵液浸出碳硅泥岩型铀矿,铀的浸出率达69.27%,铀浸出率与黑曲霉发酵液的总酸量呈正相关。When using Aspergillus niger fermentation broth to leach ore from a uranium system,the concentration of organic acids in the fermentation broth directly affects the leaching rate of uranium.In order to improve the leaching performance of Aspergillus niger fermentation broth for carbon-silica mudstone type uranium ore,orthogonal experiments were designed to optimize the acid production conditions of Aspergillus niger.The effects of pre-culture time,microparticle concentration,inoculation ratio,and fermentation culture time on the acid production of Aspergillus niger were explored.The relationship between the acid production of fermentation broth and uranium extraction rate was analyzed.The results showed that the total acid concentration in the fermentation broth of Aspergillus niger was 6880 mg/L at the optimum experimental conditions of pre-culture time of 15 h,microparticle concentration with 5 g/L,Aspergillus niger inoculation with 4%and fermentation culture time of 96 h,and thus,the uranium extraction rate reached 69.27%.The uranium extraction rate was positively correlated with the total acid in Aspergillus niger fermentation broth.

关 键 词：黑曲霉 碳硅泥岩型铀矿 发酵液浸出 有机酸浓度 

分 类 号：TF18[冶金工程—冶金物理化学]