Mrhox3影响罗伯茨绿僵菌产孢和抗逆能力的研究  

Mrhox3 Effects on Conidiation and Tolerance in Metarhizium robertsii

作　　者：张楠[1,2] 吴浩 童友敏[1] 李晓娟 黄勃 ZHANG Nan;WU Hao;TONG Youmin;LI Xiaojuan;HUANG Bo(Anhui Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036,China;College of Resources and Environment,Anhui Agricultural University,Hefei 230036,China;College of Life Sciences,Anhui Agricultural University,Hefei 230036,China;Key Laboratory of State Forestry Administration on Prevention and Control of Pine Wood Nematode Disease,Anhui Academy of Forestry,Hefei 230088,China)

机构地区：[1]微生物防治安徽省重点实验室,安徽农业大学,合肥230036 [2]安徽农业大学资源与环境学院,合肥230036 [3]安徽农业大学生命科学学院,合肥230036 [4]松材线虫病预防与控制技术国家林草局重点实验室,安徽省林业科学研究院,合肥230088

出　　处：《中国生物防治学报》2023年第3期523-532,共10页Chinese Journal of Biological Control

基　　金：国家自然科学基金(32172473,31972332)。

摘　　要：罗伯茨绿僵菌作为一种昆虫病原真菌,已在害虫生物防治中得到应用。Homeobox家族基因在真菌无性繁殖过程中具有重要作用,但是其在昆虫病原真菌中的功能尚不清楚。qRT-PCR结果显示homeobox家族基因Mrhox3在产孢阶段和分生孢子阶段高水平表达。通过基因敲除与回补研究其功能,结果表明敲除Mrhox3不影响菌株营养生长和致病性,但是显著降低菌株的产孢能力。进一步研究显示敲除Mrhox3显著下调关键调控因子abaA和wetA等多个产孢相关基因的表达水平。敲除Mrhox3促进分生孢子萌发,并显著增强分生孢子对紫外胁迫和热激胁迫的耐受力。本研究将为进一步揭示罗伯茨绿僵菌的产孢机制和分生孢子抗逆机制奠定基础。Metarhizium robertsii,as a model entomopathogenic fungus,has been applied in biocontrol programs of insect pests.Homeobox family genes play an important role in fungal asexual reproduction,however,its functions in entomopathogenic fungi remains unclear.Quantitative real-time PCR showed that homeobox family gene Mrhox3 is highly expressed at the conidiation and conidia stages.Gene knock-out and complementation were conducted to study the function of Mrhox3,and the results indicated that deletion of Mrhox3 did not affect the mutant’s vegetative growth and pathogenicity,but significantly weakened the conidiation ability.Further studies showed that loss of Mrhox3 significantly down-regulated the expression levels of multiple conidiation-related genes,such as the centarl regulators abaA and wetA.Loss of Mrhox3 resulted in promoted conidial germination and significantly enhanced conidial tolerance to UV and heat shock stress.This study provides a foundation for further understanding the mechanism of conidiation and conidia stress resistance in Metarhizium robertsii.

关 键 词：罗伯茨绿僵菌 Mrhox3 产孢 逆境胁迫 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]