白术疫霉拮抗链霉菌的筛选、鉴定及其抑菌效果  

Screening,Identification and Antagonistic Effect of Streptomyces against Phytophthora sansomeana

作　　者：李文巧 王娇 刘晓宁 李亚宁[1] 何运转[1] 刘大群[1] LI Wenqiao;WANG Jiao;LIU Xiaoning;LI Yaning;HE Yunzhuan;LIU Daqun(National Engineering Research Center for Agriculture in Northern Mountainous Areas/Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province/College of Plant Protection,Hebei Agricultural University,Baoding 071001,China)

机构地区：[1]河北农业大学植物保护学院/河北省农作物病虫害生物防治技术创新中心/国家北方山区农业工程技术研究中心,保定071001

出　　处：《中国生物防治学报》2023年第3期636-645,共10页Chinese Journal of Biological Control

基　　金：国家自然科学面上基金(32272605);河北省现代农业产业技术体系创新团队(HBCT2018060204);河北省省级科技计划项目(20567672H)。

摘　　要：本文从河北省安国市白术疫病发生地块的健康白术植株的根际土壤中,分离筛选对白术疫霉Phytophthora sansomeana有拮抗作用的链霉菌菌株,旨在为白术疫霉病害的绿色防控提供有效的生防资源。采用稀释涂布法和平板对峙法,分离、筛选得到3株对白术疫霉拮抗效果较好的链霉菌菌株BZX-23、BZX-31、BZX-104。经16S rDNA序列分析,结合菌落形态特征、生理生化特征,将这3株拮抗链霉菌菌株分别鉴定为加利利链霉菌Streptomyces galilaeus、包比链霉菌S.bobili、苜蓿链霉菌S.alfalfae,其对白术疫霉的抑制作用均在60%以上。To provide effective biological control resources for the green control of P.sansomeana,Streptomyces strains were isolated and screened with antagonistic effects against P.sansomeana from rhizosphere soil of healthy atractylodes plants in the area of P.sansomeana in Anguo of Hebei province.As results,three antagonistic Streptomyces strains(BZX-104,BZX-23 and BZX-31)with good antagonistic effect against P.sansomeana were isolated and screened by dilution coating method and plate confrontation method.After 16S rDNA sequence analysis,combined with colony morphological characteristics,and physiological and biochemical characteristics,the three antagonistic strains were identified as S.alfalfae,S.galilaeus and S.bobili,respectively.Their inhibitory activity against P.sansomeana were more than 60%.

关 键 词：白术疫霉 拮抗链霉菌 16S rDNA序列分析 抑菌效果 

分 类 号：S476[农业科学—农业昆虫与害虫防治]