短距乌头中三个新的C_(19)-二萜生物碱  

Three New C_(19)-Diterpenoid Alkaloids from Aconitum brevicalcaratum

作　　者：邢凤 曾浩 李茜 万丹 丁欢欢 谢江 陈琳[1] 周先礼[1,2,3] Xing Feng;Zeng Hao;Li Qian;Wan Dan;Ding Huanhuan;Xie Jiang;Chen Lin;Zhou Xianli(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031;Key laboratory of Advanced Technologies of Materials,Ministry of Education,Southwest Jiaotong University,Chengdu 610031;Affiliated Hospital of Southwest Jiaotong University&The Third People Hospital of Chengdu,Chengdu 610031)

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]西南交通大学教育部材料先进技术重点实验室,成都610031 [3]西南交通大学附属医院成都第三人民医院,成都610031

出　　处：《有机化学》2023年第6期2245-2251,共7页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.82073734);四川省应用基础(No.2021YJ0222);四川省中医药管理研究基金(No.2020HJZX002);中央高校基本科研业务费专项(No.2682021CX085);大学生创新创业培训计划(No.202210613076)资助项目.

摘　　要：从短距乌头中分离得到3个新的C_(19)-二萜生物碱和7个已知化合物,通过高分辨率质谱(HR-ESI-MS)、红外吸收光谱法(IR)、一维核磁共振(1D NMR)、二维核磁共振(2D NMR)和X单晶衍射等波谱技术鉴定了其结构.Brevicalcarine A(1)是一种新的二萜生物碱,其在C-18位连接有vaginatunine A片段,brevicalcarine B(2)和brevicalcarine C(3)是一种在C-18有乙酰基的C_(19)-二萜生物碱.此外,还研究了这些化合物对BV-2细胞的抗神经炎活性,结果表明它们均不具有显著的抗神经炎活性.Three new C_(19)-diterpenoid alkaloids,brevicalcarines A~C(1~3),together with seven known compounds(4~10),were isolated from the roots of A.brevicalcaratum(Finet et Gagnep.)Diels.Their structures were determined by HR-ESI-MS(high resolution electrospray ionization mass spectroscopy),IR(Infrared absorption spectroscopy),1D NMR(One-dimensional nuclear magnetic resonance),2D NMR(Two-dimensional nuclear magnetic resonance)and X-ray crystallographic analysis.Brevicalcarine A(1)was a new diterpenoid alkaloid with vaginatunine A fragment at C-18,while brevicalcarine B(2)and brevicalcarine C(3)were new C_(19)-diterpenoid alkaloid with an acetoxy group at C-18.In addition,the anti-neuroinflammatory activity of these compounds against BV-2 cells was investigated,which showed that none of them had significant anti-neuroinflammatory activity.

关 键 词：毛茛科 短距乌头 二萜生物碱 抗神经炎症活性 

分 类 号：TQ460.1[化学工程—制药化工]