复肝汤HPLC特征图谱及波长切换法同时测定6种成分的含量  

Characteristic fingerprints and simultaneous determination of 6 constituents in Fugan decoction by HPLC wavelength switching method

作　　者：袁文钦 黄伟鹏 林雅丽 段崇申 刘怡馨 陈乃宏[2] 刘应蛟 YUAN Wen-qin;HUANG Wei-peng;LIN Ya-li;DUAN Chong-shen;LIU Yi-xin;CHEN Nai-hong;LIU Ying-jiao(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004;Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050)

机构地区：[1]江西中医药大学药学院,南昌330004 [2]中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《中南药学》2023年第8期2158-2162,共5页Central South Pharmacy

基　　金：江西省中医药管理局科技计划项目(No.2021A333);江西省卫生健康委科技计划(No.202211412);江西中医药大学校级教改课题(No.2021jzyb-14);江西中医药大学校级科技创新团队发展计划(No.CXTD22002)。

摘　　要：目的建立复肝汤HPLC特征图谱,并测定复肝汤中毛蕊花糖苷、芦丁、槲皮素、木犀草素、芹菜素、丹参酮Ⅱ_(A)6种主要成分的含量。方法采用高效液相色谱法,色谱柱为Agilent 5TC-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水,梯度洗脱,流速为1.0 mL·min^(-1),检测波长分别为310 nm(毛蕊花糖苷)、370 nm(芦丁、槲皮素、木犀草素、芹菜素)、250 nm(丹参酮Ⅱ_(A)),柱温30℃,进样量20μL。结果确定复肝汤HPLC特征图谱13个共有峰,利用相似度软件对10批样品特征图谱进行分析,各批样品相似度均在0.95以上。毛蕊花糖苷、芦丁、槲皮素、木犀草素、芹菜素和丹参酮ⅡA的线性范围分别在4.394~87.880μg·mL^(-1)(r=0.9999)、1.501~30.020μg·mL^(-1)(r=0.9999)、0.475~9.505μg·mL^(-1)(r=0.9997)、0.547~10.945μg·mL^(-1)(r=0.9995)、1.028~20.550μg·mL^(-1)(r=0.9998)、0.854~17.070μg·mL^(-1)(r=1.000);平均加样回收率在92.1%~101.3%,RSD在0.78%~1.4%。结论该方法简便、准确、重复性高,并可同时测定复肝汤中6种成分的含量,为复肝汤质量控制提供了参考。Objective To simultaneously determine the content of verbascoside,rutin,quercetin,luteolin,apigenin and tanshinoneⅡ_(A) in Fugan decoction by HPLC and establish the HPLC fingerprints.Methods Agilent 5 TC-C_(18) column(4.6 mm×250 mm,5μm)was used with the mobile phase consisting of methyl alcohol-water in a gradient elution at 1.0 mL·min-1.The detection wavelengths was 310 nm(for verbascoside),370 nm(for rutin,quercetin,luteolin and apigenin),and 250 nm(for tanshinoneⅡ_(A)).The column temperature was 30℃and the injection volume was 20μL.Results Totally 13 common peaks of HPLC characteristic chromatogram of Fugan decoction were determined.Similarity software was used to analyze the feature maps of 10 batches of samples.The similarity of each batch of samples was higher than 0.95.The above mentioned 6 main ingredients showed linearity at 4.394~87.880μg·mL^(-1)(r=0.9999),1.501~30.020μg·mL^(-1)(r=0.9999),0.475~9.505μg·mL^(-1)(r=0.9997),0.547~10.945μg·mL^(-1)(r=0.9995),1.028~20.550μg·mL^(-1)(r=0.9998),and 0.854~17.070μg·mL^(-1)(r=1.000),respectively.The average recoveries ranged from 92.1%to 101.3%,with RSD at 0.78%~1.4%.Conclusion The method is simple,accurate and reproducible,and can simultaneously determine the content of 6 components in Fugan decoction,which provides the experimental basis for the quality control of Fugan decoction.

关 键 词：高效液相色谱法 复肝汤 毛蕊花糖苷 芦丁 槲皮素 木犀草素 芹菜素 丹参酮Ⅱ_(A) 

分 类 号：R284.1[医药卫生—中药学]