基于Aβ42转基因果蝇对红参多糖防治阿尔茨海默病作用及机制的研究  

Efficacy and Mechanism of Polysaccharides in Ginseng Radix et Rhizoma Rubra in Treating Alzheimer′s Disease in Aβ42 Transgenic Drosophila

作　　者：白玉莹 徐新房 张姝妍 程水清 王璇 文佳 李春帅 徐文娟 杨琳琳 李向日[1] BAI Yuying;XU Xinfang;ZHANG Shuyan;CHENG Shuiqing;WANG Xuan;WEN Jia;LI Chunshuai;XU Wenjuan;YANG Linlin;LI Xiangri(Centre of TCM Processing Research,Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药炮制研究中心/中药品质评价北京重点实验室,北京102488

出　　处：《世界中医药》2023年第15期2148-2153,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(81973480);国家重点研发计划中医药现代化研究重点专项(2019YFC1711500)。

摘　　要：目的:观察红参多糖对β淀粉样蛋白(Aβ)42转基因果蝇阿尔茨海默病模型行为学的影响,并检测果蝇脑组织β淀粉样蛋白1-42(Aβ1-42)、超氧化物歧化酶(SOD)、丙二醛(MDA)、环腺苷酸(cAMP)及乙酰胆碱酯酶(AChE)水平,评价红参多糖对阿尔茨海默病的治疗作用并探讨其可能的作用机制。方法:正常野生型果蝇作为空白组,Aβ42转基因果蝇随机分为3组,即模型组、红参多糖组、盐酸多奈哌齐阳性对照组。8 d后比较果蝇的攀附指数、进入无障碍可食离心管果蝇的个数以及果蝇脑组织中Aβ1-42、SOD、MDA、cAMP及AChE含量。结果:攀附指数显示红参多糖组、阳性对照组均极显著高于模型组,差异均有统计学意义(均P<0.01),且红参多糖组与阳性对照组比较,差异无统计学意义(P>0.05);在各时段内,红参多糖组、阳性对照组进入无障碍可食离心管果蝇个数均显著高于模型组,差异有统计学意义(P<0.05或P<0.01)。红参多糖组、阳性对照组果蝇SOD活性均显著高于模型组,差异均有统计学意义(均P<0.01),红参多糖组、阳性对照组果蝇Aβ1-42、MDA、cAMP、AChE含量均显著低于模型组,差异均有统计学意义(均P<0.01),且红参多糖组与阳性对照组比较,差异无统计学意义(P>0.05)。结论:红参多糖可提高果蝇脑组织中SOD活性、降低其Aβ1-42、MDA、cAMP及AChE水平,推测红参多糖可能是通过改善Aβ沉积、抗氧化和改善神经细胞信号转导等方面,对Aβ42转基因果蝇起到较好的治疗作用,且其效果与阳性对照药相当。Objective:To observe the effects of polysaccharides in Ginseng Radix et Rhizoma Rubra on the behaviors and the levels of amyloid-beta 42(Aβ42),superoxide dismutase(SOD),malondialdehyde(MDA),cyclic adenosine monophosphate(cAMP),and acetylcholin esterase(AChE)in the brain issue of the Aβ42 transgenic Drosophila model of Alzheimer′s disease,and explore the efficacy and mechanism of the polysaccharides in treating Alzheimer′s disease.Methods:Wild-type(Canton-S)Drosophila was used as the blank group.Aβ42 transgenic Drosophila individuals were randomized into model,polysaccharides,and donepezil hydrochloride(positive control)groups.After 8 days,the climbing index,the number of fruit flies entering the barrier-free centrifuge tube,and the levels of Aβ1-42,SOD,MDA,cAMP,and AChE in the brain issue were compared.Results:The climbing index in the polysaccharides and positive control groups were higher than that in the model group(P<0.01),while the index showed no significant difference between the polysaccharide group and the positive control group(P>0.05).In each time period,the number of fruit flies entering the barrier-free centrifuge tube in the polysaccharides and positive control groups was higher than that in the model group(P<0.05 or P<0.01).The polysaccharide and positive control groups had higher SOD activity and lower levels of Aβ1-42,MDA,cAMP,and AchE than the model group(P<0.01),while there were no significant differences between the polysaccharides group and the positive control group(P>0.05).Conclusion:The polysaccharides in Ginseng Radix et Rhizoma RubraRed can increase the SOD activity and lower the Aβ1-42,MDA,cAMP,and AChE levels in the brain tissue of Drosophila.The polysaccharides may exert the therapeutic effect by inhibiting Aβ1-42 deposition,anti-oxidation,and improving nerve cell signal transduction,with the efficacy comparable to that of the positive control.

关 键 词：红参多糖 阿尔茨海默病 Aβ42转基因果蝇 行为学 抗氧化 认知障碍 

分 类 号：R284[医药卫生—中药学] R282[医药卫生—中医学]