玉米籽粒突变体crk4的基因克隆与等位性分析  

Map-based cloning and allelic analysis of gene controlling maize kernel mutant crk4

作　　者：李萌园 张文成 高勇 秦永田 薄仕榕 宋琨洋 汤继华 付志远 LI Meng-Yuan;ZHANG Wen-Cheng;GAO Yong;QIN Yong-Tian;BO Shi-Rong;SONG Kun-Yang;TANG Ji-Hua;FU Zhi-Yuan(National Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops/College of Agronomy,Henan Agricultural University,Zhengzhou 450046,Henan,China;Hebi Academy of Agricultural Sciences,Hebi 458030,Henan,China)

机构地区：[1]河南农业大学农学院/省部共建小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心,河南郑州450046 [2]鹤壁市农业科学院,河南鹤壁458030

出　　处：《作物学报》2023年第10期2613-2620,共8页Acta Agronomica Sinica

基　　金：河南省重点研发与推广专项(科技攻关)项目(232102111080)资助。

摘　　要：籽粒突变体是克隆籽粒发育关键基因并解析其遗传调控机制的重要材料。crk4(crumpled kernel 4)是育种选系过程中发现的籽粒突变体,与野生型相比,其籽粒灌浆差、粒重和发芽率显著降低。遗传分析表明该突变由单个隐性核基因控制,图位克隆将该基因定位于玉米5号染色体614 kb的物理区间内,该区间包含11个在籽粒中表达的蛋白编码基因。序列分析表明,Zm00001d017427基因的第2个外显子上存在一个由C碱基缺失造成的crk4特异的终止突变。Zm00001d017427编码金属-烟酰胺转运蛋白(Metal-nicotianamine transporter,Sh4-shrunken4/YSL2),是已报道的籽粒突变体ysl2的等位基因。等位性测验结果表明,crk4是sh4/ysl2的一个新的等位突变体。crk4的鉴定为阐明Sh4基因调控玉米籽粒发育的分子机制提供了新的种质资源。Kernel mutants are the important materials for cloning genes related to grain development and analyzing their genetic regulation mechanism.Crk4(crumpled kernel 4)is a kernel mutant identified in the course of maize breeding and selection.Compared with the wild type,crk4 showed significantly lower in grain filling,grain weight,and germination rate.Genetic analy-sis showed that the mutant was controlled by a single recessive nuclear gene,which was mapped to a 614 kb physical distance on chromosome 5 by map-based cloning.In the 614 kb interval,11 protein-coding genes were expressed in kernel.Sequence analysis revealed that there was a crk4-specific termination mutation in the second exon of Zm00001d017427 gene,which is caused by the deletion of C base.Zm00001d017427 encoded the metal-nicotianamine transporter(Sh4-shrunken4/Ysl2),which had been reported as the target gene of kernel mutant ysl2.The allelism test indicated that crk4 was a new allele mutant of ysl2/sh4.The identification of crk4 provided a new germplasm for elucidating the molecular regulation mechanism of Sh4 on seed development in maize.

关 键 词：玉米 籽粒突变体 crk4 图位克隆 sh4/ysl2 

分 类 号：S513[农业科学—作物学]