割手密转录因子SsW RKY1提高甘蔗品种抗旱能力的功能鉴定  

Functional identification of Saccharum spontaneum transcription factor SsWRKY1 to improve drought tolerance in sugarcane

作　　者：沈庆庆 王天菊 王俊刚[3] 张树珍[3] 赵雪婷 何丽莲[1] 李富生[1,4] SHEN Qing-Qing;WANG Tian-Ju;WANG Jun-Gang;ZHANG Shu-Zhen;ZHAO Xue-Ting;HE Li-Lian;LI Fu-Sheng(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,Yunnan,China;College of Resources Environment and Chemistry,Chuxiong Normal University,Chuxiong 675000,Yunnan,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China;Key Laboratory for Crop Production and Smart Agriculture of Yunnan Province,Yunnan Agricultural University,Kunming 650201,Yunnan,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]楚雄师范学院资源环境与化学学院,云南楚雄675000 [3]中国热带农业科学院热带生物技术研究所,海南海口571101 [4]云南农业大学云南省作物生产与智慧农业重点实验室,云南昆明650201

出　　处：《作物学报》2023年第10期2654-2664,共11页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31960451);国家重点研发计划项目(2018YFD1000503);云南省作物生产与智慧农业重点实验室专项(202105AG070007);云南省重大科技专项(202202AE090021);云南省教育厅科学研究基金项目(2020Y203)资助。

摘　　要：SsWRKY1是甘蔗属野生种割手密WRKY家族成员基因,开展SsWRKY1基因的功能分析,为研究SsWRKY1参与干旱调控的分子机制提供有价值的信息。本研究利用农杆菌介导转化技术获得SsWRKY1过表达株系和RNAi干扰株系,PEG干旱胁迫处理发现过表达株系受干旱胁迫伤害程度明显轻于非转基因植株,而干扰表达株系则表现严重的干旱胁迫伤害;过表达株系脯氨酸(Pro)含量显著增加,丙二醛(MDA)含量显著下降,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等抗氧化酶活性出现不同程度的提高,且胁迫响应基因MAPK级联信号传递基因MAPK、ABA生物合成基因NCED、脯氨酸生物合成基因P5CS、以及ROS清除系统基因SOD、POD、CAT的表达水平明显升高,说明过表达SsWRKY1能够提高甘蔗应对干旱胁迫的能力。本研究表明,SsWRKY1基因通过激活抗氧化系统和调节胁迫响应基因的方式增强了甘蔗对干旱的抗性,可作为甘蔗抗性遗传改良的重要基因资源。SsWRKY1 is a member gene of the WRKY family in Saccharum spontaneum.The functional analysis provides valuable information for studying the molecular mechanism of SsWRKY1 involved in drought regulation.In this study,SsWRKY1 overex-pression lines and RNAi interference expression lines were obtained by Agrobacterium-mediated transformation technology.The results showed that overexpression lines were significantly less damaged by drought stress than non-transgenic plants,while the interference expression lines showed severe drought stress damage with PEG to simulate drought stress.The content of proline in the overexpression lines increased significantly,the content of malondialdehyde decreased significantly,and the activities of anti-oxidant enzymes such as superoxide dismutase,peroxidase,and catalase increased to varying degrees.The relative expression levels of MAPK Cascade signal transduction gene MAPK,ABA biosynthesis gene NCED,proline biosynthesis gene P5CS,ROS scavenging system gene SOD,POD,and CAT were significantly increased,indicating that SsWRKY1 overexpression could im-prove the ability of sugarcane to cope with drought stress.In conclusion,SsWRKY1 enhanced the drought resistance of sugarcane by activating the antioxidant system and regulating stress response genes and could be used as an important gene resource for genetic improvement of sugarcane resistance.

关 键 词：WRKY转录因子 转基因甘蔗 抗旱性 功能鉴定 

分 类 号：S566.1[农业科学—作物学]