电针心包经穴对脑缺血大鼠不同时点脑组织Nogo-A及NgR1蛋白表达的影响  

Effects of Stimulating Acupoints of Pericardium Meridian with Electroacupuncture on Nogo-A and NgR1 Protein Expressions in Brain Tissue of Cerebral Ischemia Rats at Different Time Points

作　　者：肖豆 马文娟 唐雅妮 谢峥嵘 潘江[4] 刘民权 章薇[4] 陈成[4] XIAO Dou;MA Wenjuan;TANG Yani;XIE Zhengrong;PAN Jiang;LIU Minquan;ZHANG Wei;CHEN Cheng(Yangpu Hospital,Tongji University,Shanghai 200090,China;Changsha Hospital of Traditional Chinese Medicine,Changsha Eighth Hospital,Changsha 410100,China;Changsha Social Work College,Changsha 410004,China;The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]同济大学附属杨浦医院,上海200090 [2]长沙市中医医院,长沙市第八医院,湖南长沙410100 [3]长沙民政职业技术学院,湖南长沙410004 [4]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国中医药信息杂志》2023年第8期113-119,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81704184、82105002);湖南省自然科学基金(2022JJ40329、2022JJ30456);湖南中医药大学一流学科开放基金(2018YXJS09);湖南中医药大学第一附属医院中医康复服务能力项目(KF201905);同济大学附属杨浦医院晨光计划(Ye1202107);湖南省中医药管理局项目(D2022008)。

摘　　要：目的探究电针心包经穴对大鼠脑缺血后神经修复的影响及作用机制。方法150只SD大鼠随机分为正常组、假手术组、模型组、心包经组和肺经组,以颈外动脉插入线栓法制备大脑中动脉栓塞(MCAO)大鼠模型。心包经组选取“天泉”“曲泽”“内关”“大陵”,肺经组选取“天府”“尺泽”“列缺”“太渊”,每日电针1次,干预6 d休息1 d,分别于3、7、14、21、28 d进行取材。HE染色观察大鼠缺血侧脑组织病理改变,免疫组化染色和Western blot检测脑组织髓鞘相关生长抑制因子A(Nogo-A)、Nogo受体1(NgR1)蛋白表达。结果与正常组、假手术组比较,模型组大鼠各时点缺血侧脑组织神经细胞消失或皱缩,细胞间距增大,Nogo-A和NgR1蛋白表达明显升高(P<0.01);与模型组比较,心包经组和肺经组大鼠缺血侧脑组织神经细胞数量增加,形态结构改善,各时点Nogo-A和NgR1蛋白表达明显降低(P<0.01,P<0.05);与肺经组比较,心包经组大鼠缺血侧脑组织Nogo-A和NgR1蛋白表达降低更明显(P<0.01,P<0.05)。结论电针心包经穴能在一定程度上调节MCAO模型大鼠神经轴突发芽和再生微环境,其机制与抑制不同时点缺血脑组织Nogo-A和NgR1蛋白表达有关。Objective To explore the effect and mechanism of nerve repair by electroacupuncture at acupoints of pericardium meridian after cerebral ischemia in rats.Methods Totally 150 SD rats were randomly divided into normal group,sham-operation group,model group,pericardial meridian group and lung meridian group.A rat model of middle cerebral artery occlusion(MCAO)was established by inserting a suture into the external carotid artery.The pericardium meridian group selected“Tianquan”(PC2),“Quze”(PC3),“Neiguan”(PC6)and“Daling”(PC7),and the lung meridian group selected“Tianfu”(LU3),“Chize”(LU5),“Lieque”(LU7)and“Taiyuan”(LU9).Electroacupuncture was performed once a day,6 days of intervention and 1 day of rest,materials were collected on 3,7,14,21,and 28 day of intervention,respectively.Pathological changes of ischemic brain tissue in rats were observed by HE staining,Nogo-A and NgR1 protein expressions in brain tissue were detected by immunohistochemical staining and Western blot.Results Compared with the normal group and the sham-operation group,the nerve cells in ischemic brain tissue of the rats in model group disappeared or shrunken at each time point,the distance between the cells increased,and the expressions of Nogo-A and NgR1 proteins significantly increased(P<0.01).Compared with the model group,the number of nerve cells in ischemic brain tissue of the rats in pericardium meridian group and lung meridian group increased,the morphological structure was improved,and the protein expressions of Nogo-A and NgR1 protein at each time point significantly decreased(P<0.01,P<0.05).Compared with the lung meridian group,the expressions of Nogo-A and NgR1 proteins in ischemic brain tissue of the pericardium meridian group significantly decreased(P<0.01,P<0.05).Conclusion Electroacupuncture stimulation at acupoints of pericardium meridian can regulate the neural axon sprouting and regeneration microenvironment in MCAO model rats,and its mechanism is related to the inhibition of Nogo-A and NgR1 protei

关 键 词：脑缺血 心包经穴 电针 神经生长抑制因子 大鼠 

分 类 号：R245.3[医药卫生—针灸推拿学]