Studies on culture condition of new marine bacterium Zooshikella sp. SY01  

作　　者：Wenjian LAN Linfeng MO Chuanghua CAI Yipin ZHOU Junhua YAO Houjin LI 

机构地区：[1]School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510080,China [2]School of Chemistry and Chemical Engineering,Sun Yat-sen University,Guangzhou 510275,China [3]South China Sea Institute of Oceanology,The Chinese Academy of Sciences,Guangzhou 510301,China [4]Instrumental Analysis and Research Center,Sun Yat-sen University,Guangzhou 510275,China

出　　处：《Frontiers of Chemical Science and Engineering》2008年第4期443-446,共4页化学科学与工程前沿（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.20502036,20602044);the Natural Science Foundation of Guangdong Province(05300667);the Young Teacher Research Foundation of Sun Yat-sen University(2004-36000-1131072).

摘　　要：New marine bacterium Zooshikella sp.SY01,producer of prodigiosin,was isolated from the seawaters of Sanya Bay.The culture conditions of this bacterium were investigated.Zooshikella sp.SY01 was cultured in 2216E media which contained tryptophan,histidine,lac-tonic acid,camphor,limonene,casein,diphenyl guani-dine,coumarin and 1,3-dinitrobenzene,respectively.After 5 days cultivation,the extracts of different culture broths were detected by direct infusion mass spectroscopy using positive ESI mode.As the results,tryptophan,his-tidine and casein didn't show any observable influences on the biosynthesis of prodigiosin.Lactonic acid,camphor,limonene,diphenyl guanidine,coumarin could inhibit the bacterium growth and prodigiosin biosynthesis to a cer-tain extent,slower the culture broth to turn red.However,1,3-dinitrobenzene inhibited the bacteria to produce pro-digiosin completely.MS data suggested that various metabolites with chemodiversity were produced in differ-ent culture media.In particular,a series of high-molecu-lar-weight compounds with high relative abundances were observed in the medium containing limonene.To further optimize the culture condition,more new prodigiosin ana-logues and lead compounds can be obtained and the goal of"one strain-many compounds"can be achieved.

关 键 词：marine bacterium Zooshikella sp.SY01 cul-ture PRODIGIOSIN 

分 类 号：O62[理学—有机化学]