Separation of epigallocatechin-3-gallate from crude tea polyphenols by using Cellulose diacetate graft β-cyclodextrin copolymer asymmetric membrane  

作　　者：Hong ZHU Peiyong QIN 

机构地区：[1]Beijing Key Laboratory of Bioprocess,Beijing University of Chemical Technology,Beijing 100029,China

出　　处：《Frontiers of Chemical Science and Engineering》2011年第3期330-338,共9页化学科学与工程前沿（英文版）

基　　金：gratefully express their appreciation for the financial support obtained from the National Natural Science Foundation of China(Grant No.20636010,20876011 and 20606006);the National Basic Research Program of China(Grant No.2007CB714304);the National High Technology Research and Development Program of China(Grant Nos.2007AA100404,2007AA10Z360);the Beijing key laboratory of bioprocesses.

摘　　要：This study demonstrates a new Cellulose diacetate graft b-cyclodextrin(CDA-b-CD)copolymer asymmetric membrane prepared by a phase inversion technique for the separation of(–)-epigallocatechin-3-gallate(EGCG)from other polyphenols in crude tea.The graft copolymer,CDA-b-CD,was synthesized by pre-polymerization of cellulose diacetate(CDA)and 1,6-hexamethylene-diisocyanate(HDI),which was then grafted with b-cyclodextrin(b-CD).Surface and cross-section morphologies of the CDA-b-CD membranes were analyzed by using scanning electron microscopy(SEM).Fourier transform infrared spectroscopy(FT-IR)indicated that the b-CD was grafted onto the CDA by chemical bonding.The influences of the HDI/CDA mass ratio and the catalyst mass fraction on the b-CD graft yield were investigated.The optimum conditions of a HDI/CDA mass ratio of 0.35 g$g–1 and a catalyst mass fraction of 0.18 wt-%produced ab-CD graft yield of 26.51 wt-%.The effects of the b-CD graft yield and the concentration of the polymer cast solution on the separation of EGCG were also investigated.Under optimum conditions of a b-CD graft yield of 24.21 wt-%and a polymer concentration of 13 wt-%,the purity of EGCG increased from 26.51 to 86.91 wt-%.

关 键 词：(-)-epigallocatechin-3-gallate(EGCG) tea polyphenols CDA-β-CD 

分 类 号：O63[理学—高分子化学]