麦冬皂苷D对小鼠力竭游泳抗疲劳作用的研究  

Antifatigue effect of ophiopogonin D on exhaustive swimming in mice

作　　者：沈宁宁 刘昱甫 茹毅 师卓 杨春启 倪喆鑫 肖成荣 马增春 王宇光 高月[1,2] SHEN Ningning;LIU Yufu;RU Yi;SHI Zhuo;YANG Chunqi;NI Zhexin;XIAO Chengrong;MA Zengchun;WANG Yuguang;GAO Yue(School of Basic Medical Sciences,Anhui Medical University,Hefei Anhui 230032,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Jiangxi University of Chinese Medicine,Nanchang Jiangxi 330004,China)

机构地区：[1]安徽医科大学基础医学院,安徽合肥230032 [2]军事科学院军事医学研究院辐射医学研究所,北京100850 [3]江西中医药大学,江西南昌330004

出　　处：《中国药物警戒》2023年第7期749-753,共5页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(81873032);国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTDD-202207、ZYYCXTD-C-202009)。

摘　　要：目的通过力竭游泳构建小鼠疲劳模型,观察麦冬皂苷D的抗疲劳作用。方法本次实验共分3批进行。第1批ICR小鼠随机分为空白对照组,游泳模型组,麦冬皂苷D低(20 mg·kg^(-1))、中(40 mg·kg^(-1))和高(60 mg·kg^(-1))剂量组,在连续灌胃给予受试药物1周后,第7天通过负重游泳实验评价抗疲劳活性,次日通过非负重游泳实验测定血清肌酸激酶(CK)、甘油三酯(TRIGL)和尿素氮(UREAL)。第2、3批小鼠按上述剂量给药1周后进行非负重游泳实验,采用血乳酸检测等技术手段,分析麦冬皂苷D高、中、低剂量对小鼠体重、血乳酸、肝糖原的影响。结果与游泳模型组比较,灌胃麦冬皂苷D的小鼠负重游泳时间延长、肝糖原含量升高、血清CK水平下降、血清TRIGL含量升高、游泳后血乳酸曲线下面积下降,上述指标均有统计学意义(均P<0.05);血清UREAL下降,但无统计学差异(P>0.05)。结论麦冬皂苷D能明显增强小鼠运动耐力,并显著减少小鼠体内的乳酸堆积,增加肝糖原储备。Objective To observe the anti-fatigue effect of Ophiopogonin D by constructing a mouse model of exhaustive swimming exercise.Methods The mice were divided into three batches.The control group,swimming model group,lowdose(Ophiopogonin D 20 mg·kg^(-1)),medium-dose(Ophiopogonin D 40 mg·kg^(-1))and high-dose administration groups(Ophiopogonin D 60 mg·kg^(-1))were established.The weight-bearing swimming experiment was performed after a week of continuous intragastric administration.Serum creatine kinase,urea and triglyceride were measured via swimming test on the second day.One week after the second and third batches of mice were administered with the above dosages,the nonweight-bearing swimming experiment was conducted.The effects of high,medium and low doses of Ophiopogonin D on body weight,blood lactic acid and liver glycogen of mice were analyzed using the blood lactic acid detection kit,liver glycogen assay kit and otherwise.Results Compared with the swimming model group,the duration of weight-bearing swimming Ophiopogonin D mice was prolonged,the liver glycogen content was increased,the serum creatine kinase level was decreased,the serum triglyceride content was increased,and the area under the blood lactic acid curve was decreased after swimming.All the above indexes were of statistical significance(all P<0.05).Serum urea nitrogen decreased,but the change was not statistically significant(P>0.05).Conclusion Ophiopogonin D can significantly enhance exercise endurance,reduce lactic acid accumulation and increase glycogen reserve in mice.

关 键 词：麦冬皂苷D 抗疲劳 力竭游泳 血乳酸 肝糖原 小鼠 灌胃 

分 类 号：R969.4[医药卫生—药理学]