柞蚕丝素肽的抗氧化及美白功效评价  

Evaluation of anti-oxidation and whitening efficacy of silk fibroin peptides from tussah Antherae pernyi

作　　者：李学军 米锐[1,2] 王林美 李亚洁[1,2] 陈雪 都兴范[1,2] Xuejun Li;Rui Mi;Linmei Wang;Yajie Li;Xue Chen;Xingfan Du(Liaoning Ocean and Fisheries Science Research Institute,Dalian,Liaoning 116023,China;Dalian Institute of Biotechnology,Liaoning Academy of Agricultur Sciences,Dalian,Liaoning 116023,China;Dalian Center for Food and Drug Control and Certification,Dalian,Liaoning 116037,China)

机构地区：[1]辽宁省海洋水产科学研究院,辽宁大连116023 [2]辽宁省农业科学院大连生物技术研究所,辽宁大连116023 [3]大连市检验检测认证技术服务中心,辽宁大连116037

出　　处：《日用化学工业（中英文）》2023年第8期891-898,共8页China Surfactant Detergent & Cosmetics

基　　金：辽宁省农业科学院院长基金项目(2021QN2002);财政部和农业农村部:国家现代农业产业技术体系资助;辽宁省农科院学科建设计划(2019DD268640)。

摘　　要：开展柞蚕丝素肽的抗氧化及美白功效研究。结果显示,1号(分子量2000-5000 Da)和2号(分子量<2000 Da)样品对DPPH自由基清除率的IC50分别是1.41和3.84 mg/mL,对羟基自由基清除率的IC50分别是0.085和0.091 mg/mL,总还原力水平随着样品添加剂量的增加而升高。柞蚕丝素肽的添加对紫外氧化损伤的小鼠成纤维细胞具有保护作用,能够提高超氧化物歧化酶(SOD)活力和谷胱甘肽(GSH)含量,降低丙二醛(MDA)含量。在美白功效评价方面,1号和2号样品对酪氨酸酶抑制率的IC50分别为3.65和5.84 mg/mL,并能够降低小鼠黑素瘤细胞内黑色素合成和酪氨酸酶活性。总之,柞蚕丝素肽具有抗氧化和美白活性,且分子量较大的1号样品优于2号样品。Study on antioxidant and whitening efficacy of tussah fibroin peptides was conducted.The results show that the IC50 of DPPH free radical scavenging rate of NO.1(molecular weight 2000-5000 Da)and NO.2(molecular weight<2000 Da)samples are 1.41 and 3.84 mg/mL,respectively,and the IC50 of hydroxyl free radical scavenging rate are 0.085 and 0.091 mg/mL,respectively.The total reducing power level increases with the sample dosage.The addition of tussah fibroin peptides have a protective effect on the UV-damaged mouse fibroblasts through increasing the activity of SOD and the content of GSH,and decreasing the content of MDA.In the evaluation of whitening efficacy,the IC50 of No.1 and No.2 samples of tyrosinase inhibition effect rate are 3.65 and 5.84 mg/mL,respectively,which can inhibit melanin synthesis and tyrosinase activity in mouse melanoma cells.In conclusion,tussah silk fibroin peptides have antioxidant and whitening activity,and the NO.1 sample with larger molecular weight is better than the NO.2 sample with smaller molecular weight.

关 键 词：柞蚕丝素肽 抗氧化 抗紫外氧化损伤 美白 

分 类 号：TQ658[化学工程—精细化工]