HPLC-ELSD法测定积雪草中积雪草苷及羟基积雪草苷的含量  

Determination of asiaticoside and madecassoside in Centella asiatica(L.)Urban by HPLC-ELSD

作　　者：王虹丹 胥维昌[2] 王远[2] 孔祥文[1] 孙丽娟[2] Hongdan Wang;Weichang Xu;Yuan Wang;Xiangwen Kong;Lijuan Sun(College of Science,Shenyang University of Chemical Technology,Shenyang,Liaoning 110000,China;Shenyang Chemical Research Institute Co.,Ltd.,Shenyang,Liaoning 110000,China)

机构地区：[1]沈阳化工大学理学院,辽宁沈阳110000 [2]沈阳化工研究院有限公司,辽宁沈阳110000

出　　处：《日用化学工业（中英文）》2023年第8期976-980,共5页China Surfactant Detergent & Cosmetics

摘　　要：积雪草提取物通常被用作化妆品原料,有美白、抗氧化等功效,发挥此功效的主要物质有积雪草苷、羟基积雪草苷。采用高效液相色谱-蒸发光散射检测(HPLC-ELSD)技术,建立了测定积雪草中积雪草苷和羟基积雪草苷含量的方法。选用Unitary C18色谱柱(4.6×250 mm,5μm),以乙腈-水为流动相,梯度洗脱,流速1 mL/min,柱温为30℃,采用Agilent 1260 InfinityⅡ蒸发光散射检测器(漂移管温度70℃,载气流速1.6 L/min)。研究表明,积雪草苷和羟基积雪草苷质量浓度分别在100~702 mg/L,105~738 mg/L范围内,峰面积与质量浓度线性关系良好,相关系数分别为0.9996和0.9997。积雪草苷和羟基积雪草苷平均加样回收率分别为101.20%,98.30%。相对标准偏差(RSD)均在3%以内。方法简单,准确度高,分离效果好,干扰少,可用于积雪草中积雪草苷和羟基积雪草苷的含量测定。Centella asiatica(L.)Urban extract is usually used as a cosmetic raw material,which has whitening,antioxidant and other effects.The main substances to play this effect are asiaticoside and madecassoside.In this paper,a high performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD)technique was used to determine the content of asiaticoside and madecassoside in Centella asiatica(L.)Urban.Unitary C18 column(4.6×250 mm,5μm)with acetonitrile-water as the mobile phase was used for gradient elution at a flow rate of 1 mL/min.Column temperature was 30℃.Agilent 1260 InfinityⅡdetector(drift tube temperature was 70℃)was used for column elution with the carrier gas flow rate at 1.6 L/min.The results show that the concentrations of asiaticoside and madecassoside are 100-702 mg/L and 105-738 mg/L,respectively.The linear relationship between peak area and concentration is good,and the correlation coefficients are 0.9996 and 0.9997,respectively.The average recoveries of asiaticoside and madecassoside are 101.20%and 98.30%,respectively.The relative standard deviations(RSD)are all within 3%.The method is simple,accurate,effective and less interference,and can be used for the determination of asiaticoside and madecassoside in Centella asiatica(L.)Urban.

关 键 词：积雪草 高效液相色谱-蒸发光散射检测器 积雪草苷 羟基积雪草苷 含量测定 

分 类 号：TQ658[化学工程—精细化工]