二妙丸中黄柏薄层色谱鉴别方法的改进  

Improvement of the Thin Layer Chromatography Identification for Huangbai in ErMiao Pill Engineering Specialized Subject

作　　者：陈帅[1,2] 王亮 徐畅 蒋玉欣 王慧竹[1] Chen Shuai;Wang Liang;Xu Chang;Jiang Yuxin;Wang Huizhu(School of Chemistry and Pharmaceutical EngineeringJilin Institute of Chemical Technology,Jilin 132022;Graduate School of Jilin Institute of Chemical Technology,Jilin 132022,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022 [2]吉林化工学院研究生院,吉林吉林132022

出　　处：《广东化工》2023年第13期188-190,共3页Guangdong Chemical Industry

基　　金：2018年吉林化工学院高等教育教学研究课题(重点项目)。

摘　　要：目的:改进二妙丸中黄柏薄层色谱鉴别方法。方法:以盐酸小檗碱为对照品,考察不同展开剂、展开剂比例、点样量、环境温度、环境湿度对二妙丸中黄柏薄层色谱分析的影响,确定二妙丸中黄柏薄层色谱分析的最佳条件。结果:采用乙酸乙酯-甲醇-浓氨试液(10∶1∶1)作为展开剂,点样量1μL,在环境温度25℃,环境湿度范围30%~70%下进行薄层色谱鉴别实验,成分斑点清晰、分离度好,且阴性对照无干扰。结论:该薄层色谱方法操作简便,专属性强,可用于二妙丸中黄柏的薄层鉴别,适合作为制药工程专业药物化学实验项目开展实验教学。Objective:Improve the identification method of thin layer chromatography for huangbai in ermiao pill.Methods:Berberine hydrochloride was used as reference substance to investigate the effects of different developing agents,the ratio of developing agent,testing dose,ambient temperature and ambient humidity on the thin-layer chromatographic analysis for huangbai in ermiao Pill,and the optimal conditions that is for the thin-layer chromatography for huangbai in ermiao pill were determined.Results:Ethyl acetate-methanol-concentrated ammonia test solution(10∶1∶1)was used as the developing agent.The testing dose was 1μL,and the thin layer chromatography identification experiment was carried out at an ambient temperature of 25℃and an ambient humidity range of 30%~70%.Under the optimal conditions,the component spots were clear,and the resolution was good.Furthermore,the negative control had no interference.Conclusion:The thin layer chromatography method is simple to operate and has strong specificity.It can be used for the identification huangbai in ermiao pill.Moreover,it is suitable to carry out as a pharmaceutical chemistry experiment project for experimental teaching in pharmaceutical engineering specialty.

关 键 词：薄层色谱 二妙丸 黄柏 盐酸小檗碱 鉴别 

分 类 号：TQ041[化学工程]