氮和生物炭添加对毛竹林AMF侵染率和孢子密度的影响  

Effects of Nitrogen and Biochar Addition on Arbuscular Mycorrhizal Fungi Colonization Rate and Spore Density in Moso Bamboo Plantations

作　　者：史雪荣 黄宏亮 季杭翔 赵明水 张华锋 李全 宋新章[1] SHI Xue-rong;Huang Hong-liang;JI Hang-xiang;ZHAO Ming-shui;ZHANG Hua-feng;LI Quan;SONG Xin-zhang(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Forest Bureau of Anji County,Huzhou 313000,Zhejiang,China;Tianmu Mountain Forest Ecosystem Research Station,Hangzhou 311300,Zhejiang,China;Agroforestry Technology Extension Center in Lin’an District,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300 [2]安吉县林业局,浙江湖州313000 [3]浙江省天目山国家级自然保护区管理局,浙江杭州311300 [4]杭州市临安区农林技术推广中心,浙江杭州311300

出　　处：《林业科学研究》2023年第4期165-172,共8页Forest Research

基　　金：国家自然科学基金(31470529,32125027)资助。

摘　　要：[目的]探讨氮沉降和生物炭添加对毛竹林丛枝菌根真菌特征的影响,为全球变化背景下毛竹林的可持续经营提供科学参考。[方法]以毛竹林为研究对象,探讨了不同梯度的氮沉降(0、30、60和90 kg·hm^(−2)·yr^(−1))和生物炭添加(0、20和40 t·hm^(−2))及其复合作用对毛竹林AMF侵染率和孢子密度以及土壤理化性质的影响。[结果]与对照(0 kg·hm^(−2)·yr^(−1) N+0 t·hm^(−2) BC)相比,氮添加显著降低了AMF侵染率(16.1%~51.7%)。高生物炭添加(40 t·hm^(−2))显著降低了AMF侵染率(46.0%),但提高了孢子密度(162.5%)。在氮添加处理下,生物炭添加提高了AMF侵染率,对孢子密度无显著影响。AMF侵染率与土壤pH呈显著正相关关系,与有效氮和有效磷呈显著负相关关系。AMF孢子密度与有效磷呈显著正相关关系。[结论]在氮添加处理下,生物炭添加提高了AMF侵染率,增强了毛竹与AMF的共生关系,表明在氮沉降背景下生物炭添加是实现毛竹林可持续经营的有效措施之一。[Objective]To investigate the effects of nitrogen(N)deposition and biochar addition on arbuscular mycorrhizal fungi(AMF)characteristics in Moso bamboo(Phyllostachys edulis(Carrière)J.Houz.)plantations for providing scientific reference for sustainable management of Moso bamboo plantations under global change.[Methods]We investigated the effects of different N addition rates(0,30,60,and 90 kg N·hm^(−2)·yr^(−1)),biochar addition rates(0,20,and 40 t BC·hm^(−2)),and their combination on the AMF colonization rate,spore density and soil properties of Phyllostachys edulis plantations.[Results]Compared with the control(0 kg N·hm^(−2)·yr^(−1)+0 t BC·hm^(−2)),N addition significantly reduced the colonization rate of AMF(16.1%~51.7%).High biochar addition(40 t BC·hm^(−2))significantly reduced the colonization rate of AMF(46.0%),but increased the spore density(162.5%).Under the N addition treatments,biochar addition increased the colonization rate of AMF,and had no significant effect on spore density.The colonization rate of AMF was significantly positively correlated with soil pH,and negatively correlated with available N and available phosphorus.The spore density of AMF was significantly positively correlated with available phosphorus.[Conclusion]Under the N addition treatments,biochar addition increased the colonization rate of AMF and enhanced the symbiotic relationship between Moso bamboo and AMF,indicating that biochar addition is an effective measure to achieve sustainable management of Moso bamboo plantations under the background of N deposition.

关 键 词：毛竹 氮沉降 生物炭 丛枝菌根真菌 侵染率 

分 类 号：S718.81[农业科学—林学]