植物病原细菌Ⅵ型分泌系统研究进展  

A Review on the TypeⅥSecretion System of Plant Pathogenic Cacteria

作　　者：夏雄飞 薄淑文 韩长志[1,2] XIA Xiong-fei;BO Shu-wen;HAN Chang-zhi(College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,Yunnan,China;The Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Kunming 650224,Yunnan,China)

机构地区：[1]西南林业大学生物多样性保护学院,云南昆明650224 [2]云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《林业科学研究》2023年第4期183-192,共10页Forest Research

基　　金：云南省“兴滇英才支持计划”青年人才专项(YNWR-QNBJ-2020-188);国家自然科学基金项目(31960314);云南省研究生导师团队建设项目“森林病理学硕士生导师团队”(2022100)。

摘　　要：[目的]Ⅵ型分泌系统(T6SS)作为植物病原细菌发挥重要功能的调控系统之一,主要表现为通过效应子的释放对宿主植物的攻击性和自身在种间竞争过程中面对胁迫反应的防御性等方面,明确重要植物病原细菌的T6SS蛋白及功能差异,有助于推动预防和治疗植物病害的新型农药的开发和利用。[方法]通过对比分析5种重要植物病原细菌(丁香假单胞菌、青枯雷尔氏菌、根癌农杆菌、梨火疫病菌、稻生黄单胞菌)中138个T6SS蛋白的保守结构域以及遗传关系,同时,基于对国内外文献数据库中有关上述5种细菌的T6SS功能研究的文献开展分析。[结果]明确5种植物病原细菌中T6SS蛋白在种类和数量上存在差异性。根据蛋白序列同源性及结构域,上述蛋白分为3大类,其中,含有VgrG、HCP等结构域的T6SS蛋白具有很好的聚类区分度,而含有TssA、TssG等结构域的T6SS蛋白则可分散在不同类别中。[结论]明确5种植物病原细菌中T6SS蛋白及功能具有一定保守性和独特性特征。该研究为进一步开展不同植物病原细菌中T6SS蛋白的预测分析,以及不同T6SS蛋白之间的关系解析提供理论基础。[Objective]TypeⅥsecretion system(T6SS)is one of the regulatory systems that plays important functions in plant pathogenic bacteria.In terms of defense and other aspects,clarifying the T6SS protein and functional differences of important plant pathogenic bacteria will help promote the development and utilization of new pesticides for the prevention and treatment of plant diseases.[Method]In the study,five kinds of important plant pathogenic bacteria(Pseudomonas syringae,Ralstonia solanacearum,Agrobacterium tumefaciens,Erwinnia amylovora and Xanthomonas oryzae pv.oryzicola)bacterium in 138 conservative T6SS proteins structure domain and genetic relationships were analyzed,and the literatures about the above five kinds of bacteria in the database of the T6SS function research were also analyzed.[Results]T6SS proteins in the five kinds of plant pathogenic bacteria were different in species and quantity.According to proteins sequence homology and domain,the above proteins could be divided into three categories.Among them,T6SS proteins containing VgrG,HCP and other domains had good clustering discrimination,while T6SS proteins containing TssA,TssG and other domains could be divided into different categories.[Conclusion]The proteins and function of T6SS in the five kinds of plant pathogenic bacteria have certain conservation and unique characteristics.This study provides a theoretical basis for further prediction and analysis of T6SS proteins in different plant pathogenic bacteria and the relationship between different T6SS proteins.

关 键 词：植物病原细菌 T6SS 结构域 功能 遗传关系 研究综述 

分 类 号：S432.1[农业科学—植物病理学]