基于GEO数据库分析脓毒症相关急性呼吸窘迫综合征患者血免疫细胞及基因芯片数据  

Analysis of blood immune cell and gene microarray data of patients with sepsis-related acute respiratory distress syndrome based GEO database

作　　者：严蕾 童朝阳 YAN Lei;TONG Chao-yang(Department of Emergency Medicine,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院急诊科,上海200032

出　　处：《复旦学报（医学版）》2023年第4期509-516,525,共9页Fudan University Journal of Medical Sciences

基　　金：复旦大学附属中山医院青年基金(2018ZSQN47)。

摘　　要：目的通过生物信息学方法分析Gene Expression Omnibus(GEO)数据库中脓毒症相关急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)的芯片表达数据,观察外周血免疫细胞变化,鉴定差异表达基因(differential expressed gene,DEG),探索脓毒症相关ARDS患者的免疫系统变化及关键作用基因。方法从GEO数据库GSE32707下载脓毒症相关ARDS患者的数据信息。应用CIBERSORT得到每个样本的22种免疫细胞的含量矩阵,分析第0天、第7天脓毒症及脓毒症相关ARDS患者外周血免疫细胞组成的变化。利用R软件筛选第7天脓毒症及脓毒症相关ARDS患者DEG,经基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析判断参与ARDS病情进展的重要信号途径,使用蛋白互作网络获得核心基因。结果第0天脓毒症相关ARDS患者的免疫细胞分布并没有明显区别,第7天脓毒症相关ARDS患者外周血里CD8+T淋巴细胞比例明显下降(P=0.009),中性粒细胞比例明显升高(P=0.022)。经多种算法取交集的方法共获得RPL5、EIF4A2、RPL15、EIF3E、SRP9、HSP90AA1、RBM25这7个核心基因参与脓毒症相关ARDS病情进展。结论随着病情进展,脓毒症相关ARDS外周血免疫细胞组成,相关基因调控会发生显著变化,其中7个关键基因可能在其中发挥重要作用。Objective To analyze the microarray expression data of sepsis-related acute respiratory distress syndrome(ARDS)in the Gene Expression Omnibus(GEO)database by bioinformatics methods.To observe the alterations of immune cells in peripheral blood,investigate differentially expressed genes(DEGs),and identify key genes in patients with sepsis related ARDS.Methods The data of sepsisrelated ARDS patients were downloaded from GEO database GSE32707.CIBERSORT was applied to obtain a matrix of the content of 22 immune cells in peripheral blood of patients with sepsis and sepsisrelated ARDS on day 0 and day 7.R software was used to analyze the DEGs of sepsis and sepsis-related ARDS patients on day 7,and gene ontology(GEO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis were used to determine the important signaling pathways involved in ARDS.Hub genes were evaluated by protein interaction network.Results There was no significant difference in the distribution of immune cells between sepsis and sepsis-related ARDS on day 0,but on day 7,the proportion of CD8+T cells in peripheral blood of sepsis-related ARDS decreased significantly(P=0.009).The proportion of neutrophils was significantly increased(P=0.022).Seven core genes,RPL5,EIF4A2,RPL15,EIF3E,SRP9,HSP90AA1,and RBM25,involved in the progression of sepsis-related ARDS.Conclusion With the progression of ARDS,the composition of peripheral blood immune cells in sepsis-related ARDS and the regulation of genes will change significantly,and seven hub genes may play an important role in this disease.

关 键 词：急性呼吸窘迫综合征(ARDS) 基因数据库 免疫细胞 核心基因 

分 类 号：R563[医药卫生—呼吸系统] Q786[医药卫生—内科学]