滇黄精及其炮制品中皂苷类成分的薄层色谱研究  

Study on Thin Layer Chromatography of Saponins in Polygonatum kingianum and Its Processed Products

作　　者：戴万生 彭凯 邱斌 贺森 顾雯 DAI Wansheng;PENG Kai;QIU Bin;HE Sen;GU Wen(College of traditional Chinese medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500

出　　处：《中国民族民间医药》2023年第15期29-32,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：云南省科技厅-云南中医药大学应用基础研究联合专项(2017FF116-017);云南省生物医药2021重大专项(202102AA310045)。

摘　　要：目的:建立滇黄精及其炮制品的薄层色谱鉴别方法,考查滇黄精炮制前后皂苷类成分的变化差异。方法:在《中国药典》2020版黄精薄层色谱法的基础上,对供试液的提取方法、展开剂比例、饱和时间、点样量等进行考察,并对滇黄精不同炮制品进行薄层分析。结果:优化方法为水饱和正丁醇超声提取总皂苷,以石油醚(60~90℃)∶乙酸乙酯∶甲酸(5∶2.5∶0.8)展开,饱和时间为10 min,点样量为6μL,5%香草醛硫酸溶液显色。黄精炮制后皂苷类成分产生一定变化。结论:本鉴别方法简单、快捷、分离效果好、斑点清晰,可用于滇黄精及其炮制品的鉴别。Objective To establish a TLC method for the identification of Polygonatum kingianum and its processed products,and to investigate the changes of saponins in Polygonatum kingianum before and after processing.Methods On the basis of TLC of Rhizoma Polygonati in Chinese Pharmacopoeia 2020,the extraction method,developing agent ratio,saturation time,the amount of sample,etc.of the test solution were investigated,and the different processed products of Polygonatum kingianum.were analyzed by TLC.Results The optimized method was water saturated n-butanol ultrasonic extraction of total saponins,and developed with petroleum ether(60-90℃):ethyl acetate:formic acid(5∶2.5∶0.8).The saturation time was 10min,the amount of sample was 6μL,and the color was developed with 5%vanillin sulfuric acid solution.After processing,saponins of Polygonatum kingianum produced certain changes.Conclusion The identification method is simple,rapid,with good separation effect and clear spots,which can be used to identify Polygonatum kingianum and its processed products.

关 键 词：滇黄精 炮制品 总皂苷 薄层色谱 

分 类 号：R284.1[医药卫生—中药学]