外施α-酮戊二酸对盐胁迫下海滨锦葵生长、碳氮磷养分积累及其计量关系的影响  

Effects of External Application ofα-Ketoglutarate on Growth,Carbon,Nitrogen and Phosphorus Accumulation and Their Stoichiometric Relationships in Kosteletzkya virginica Under Salt Stress

作　　者：吴雨露 扈嘉鑫 陈宇熙 郑炳松[1] 闫道良[1] WU Yulu§;HU Jiaxin§;CHEN Yuxi;ZHENG Bingsong;YAN DaoLiang(State Key Laboratory of Subtropical Silviculture,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China)

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室,杭州311300

出　　处：《中国农业科技导报》2023年第7期170-177,共8页Journal of Agricultural Science and Technology

基　　金：国家林业和草原局林业科技发展资助项目(KJZXSA2019034)。

摘　　要：为研究有机碳肥α-酮戊二酸(α-ketoglutaric acid,KG)对盐碱地植物生长调控及其碳(C)、氮(N)、磷(P)养分吸收利用的影响,以海滨锦葵(Kosteletzkya virginica)幼苗(具3~4片真叶)为试验材料,分别用0、5、15 g·L^(-1)NaCl溶液浇灌栽培基质,配以0、10、20 mg·L^(-1) α-酮戊二酸溶液叶面喷施,处理45 d后测定并分析各处理组盐胁迫下海滨锦葵生长及碳氮磷积累及其计量关系。结果表明,外源喷施α-酮戊二酸对盐胁迫下海滨锦葵植株的相对生长率没有明显影响,但促进了非盐胁迫下植株的总生物量;高盐(15 g·L^(-1) NaCl)胁迫下,外源喷施20 mg·L^(-1)的KG明显提高了海滨锦葵的根生物量、根冠比和比叶重;同时,20 mg·L^(-1) KG显著提高了叶片C和N含量,对高盐胁迫下的海滨锦葵来说,α-酮戊二酸对叶片C的积累更为明显。α-酮戊二酸对海滨锦葵盐胁迫下的N、P积累没有明显影响;外施喷施20 mg·L^(-1) KG可以显著提高15 g·L^(-1) NaCl处理下海滨锦葵的碳氮比(C∶N)和碳磷比(C∶P)比值,N和P的利用效率显著提高;5 g·L^(-1) NaCl处理下,施加10 mg·L^(-1) KG显著增加了海滨锦葵叶片K^(+)的含量,说明α-酮戊二酸可以促进盐胁迫下植株对K^(+)的吸收,对Na^(+)的吸收则无明显影响。相关分析表明,海滨锦葵叶片总N与叶片总P呈极显著正相关(P<0.01),与叶片N/P呈极显著负相关(P<0.01),而与叶片K^(+)/Na^(+)呈极显著正相关(P<0.01)。叶片总P含量与叶片总Na含量呈极显著负相关,与叶片K^(+)含量呈显著正相关。以上结果表明,一定水平的有机碳肥α-酮戊二酸能缓解盐胁迫对海滨锦葵生长的抑制,为盐碱地提高海滨锦葵的生物量和正常生长提供了新的施肥思路。In order to study the effects of organic carbon fertilizerα-ketoglutarate on the growth,carbon(C),nitrogen(N)and phosphorus(P)accumulation and their stoichiometry of Kosteletzkya virginica under salt stress,the seedlings of K.virginica(with 3~4 true leaves)were used as experimental materials,and the cultivation medium and soil were watered with different concentrations of salt solution(NaCl,0,5,15 g∙L^(-1))andα-ketoglutarate solution(KG,0,10,20 mg∙L^(-1))was sprayed on the leaves.After 45 d of treatment,these indexes were measured and analyzed,including the plant height,biomass,relative growth rate,root shoot ratio,specific leaf weight,total carbon(C),total nitrogen(N),total phosphorus(P),sodium(Na^(+)),potassium(K^(+))content and their stoichiometric ratio.The results showed that exogenous spraying KG did not significantly affect the relative growth rate of K.virginica plants under salt stress,but promoted the total biomass of plants under non salt stress;under high salt stress(15 g∙L^(-1) NaCl),exogenous 20 mg∙L^(-1) KG significantly increased the root biomass,root shoot ratio and specific leaf weight of K.virginica.At the same time,20 mg∙L^(-1) KG significantly increased the contents of C and N in leaves.For K.virginica under high salt stress,KG had more obvious accumulation of C in leaves.KG had no significant effect on the accumulation of N and P under salt stress.External application of 20 mg∙L^(-1) KG could significantly increase the C/N and C/P of K.virginica under 15 g∙L^(-1) NaCl treatment,and the utilization efficiency of N and P was significantly improved;under the treatment of 5 g·L^(-1) NaCl,the application of 10 mg∙L^(-1) KG significantly increased the K^(+)content of leaves,which showed that KG could promote the absorption of K^(+)under salt stress,but had no significant effect on the absorption of Na^(+).Correlation analysis showed that leaf N was significantly positively correlated with leaf P(P<0.01),negatively correlated with leaf N/P(P<0.01),and positively correlated with l

关 键 词：海滨锦葵 Α-酮戊二酸 盐胁迫 碳氮磷 计量关系 

分 类 号：S156[农业科学—土壤学]