Intra-specific relationships among Tibetan Eared-pheasants based on randomly amplified polymorphic DNA(RAPD)analysis  

作　　者：DING Wei ZHANG Zhengwang CHANG Jiang ZHANG Er WU Xiushan ZHANG Jinguo 

机构地区：[1]Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,Beijing Normal University,Beijing 100875,China [2]Beijing Zoo,Beijing 100044,China

出　　处：《Frontiers in Biology》2006年第4期394-397,共4页生物学前沿（英文版）

基　　金：This study was funded by the Key Grant of Chinese Ministry of Education(No.03012).

摘　　要：The Tibetan Eared-pheasant Crossoptilon harmani is a rare species native to China.A captive population has been established in the Beijing Zoo since 1999.In order to determine the kinship of the offsprings in 2001,randomly amplified polymorphic DNA(RAPD)was used to examine the parenthood of seven Tibetan Eared-pheasants in the Beijing Zoo.To amplify the genomic DNA of each individual,53 arbitrary primers were selected.The results of amplifications showed that 14 primers had clear and distinct RAPD patterns.Totally,226 amplified fragments were generated by RAPD in this study.Cluster analysis of the seven Tibetan Eared-pheasants indicated that all the four young birds had the same father(No.5 male).This study provides a practical method to determine the relationship of offsprings whose parents are unknown in birds.

关 键 词：Tibetan Eared-pheasant Crossoptilon harmani genetic relationship random amplified polymorphic DNA(RAPD) 

分 类 号：D67[政治法律—政治学]