Cortical reaction and solicitation mechanism in Hemibarbus labeo ovum  

作　　者：GAN Guangming ZHANG Yaoguang 

机构地区：[1]College of Life Science,Southwest Normal University,Chongqing 400715,China [2]Genetics Research Center,Southeast University,Nanjing 210009,China

出　　处：《Frontiers in Biology》2006年第4期398-406,共9页生物学前沿（英文版）

基　　金：This paper was supported by Chongqing Scien-tific Committee Project(No.2001-6630);Three Gorges Reservoir Area Environmental Key Laboratory of Ministry of Education Fund(No.03121).

摘　　要：There are 1 to 4 rows and five types of cortical alveoli in the cortex of the pallas(Hemibarbus labeo)egg.From the outer to the inner of the cortex,the diameter of the cortical alveoli decreases gradually.We found a new structure named solicitation speckle at the low latitude of the animal pole and near the micropylar apparatus between the ovum envelope and cell membrane in the zygote of fish.The solicitation speckle similar to type I cortical alveoli was very purple in H.E chromosome,and it had no obvious boundary with the ovum envelope and membrane.Moreover,no membrane around the solicitation speckle in TEM was found.In SEM,the solicitation speckle looked flocculent.During cortical reaction,the solicitation speckle played a very important function in arousing cortical reaction.Thirty-five seconds after fertilization,cortical alveoli began to break down near the low latitude of the animal pole.At the same time,the same thing happened near the micropylar apparatus before cortical reaction.Both starting points encountered and healed up at the vestibule of the micropylar apparatus.The cortical reaction that happened near the low latitude of the animal pole was another new pattern.The cortical reaction was divided into four parts that included latent period,developmental period,climactic period and declining period.In the latent period,no cortical alveoli were released.In the developmental period,a few cortical alveoli were released outside the cortex.In the climactic period,several cortical alveoli were inosculated into a big vesicle and released intensely.In the declining period,the type V cortical alveoli and the other remnant cortical alveoli were released.Five minutes after fertilization,cortical alveoli were released entirely in the animal pole.Five minutes after fertilization,all of the remnant cortical alveoli were released.This leads us to conclude that cortical reaction is induced by type I cortical alveoli,and the solicitation speckle is a volcanic chain reaction under water or the other lower osmotic pre

关 键 词：Hemibarbus labeo cortical alveoli cortical reaction solicitation speckle solicitation mechanism 

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