qRT-PCR、G试验及GM试验在侵袭性真菌感染诊断中的价值  

Value of qRT-PCR,G Test Combined with GM Test in the Diagnosis of Invasive Fungal Infection

作　　者：刘松 常青[1] 许宏 LIU Song;CHANG Qing;XU Hong(The First People's Hospital of Zhengzhou,Henan Zhengzhou 450000)

机构地区：[1]郑州市第一人民医院,河南郑州450000

出　　处：《深圳中西医结合杂志》2023年第12期73-75,共3页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:研究实时荧光定量聚合酶链式反应(qRT-PCR)、(1,3)-β-D-葡聚糖试验(G试验)和半乳甘露聚糖抗原试验(GM试验)在侵袭性真菌感染诊断中的价值。方法:选择2020年1月至2022年6月在郑州市第一人民医院就医的147例可疑侵袭性真菌感染患者为研究对象,采用真菌培养法鉴定感染组真菌类型。分别采用qRT-PCR、G试验及GM试验检测可疑侵袭性真菌感染患者样本,以真菌培养结果为标准,分析qRT-PCR、G试验及GM试验在侵袭性真菌感染诊断中的价值。结果:147例可疑侵袭性真菌感染患者中,86例检测到侵袭性真菌(阳性率58.5%),包括白色念珠菌21例(24.4%)、曲霉菌18例(20.9%)、热带念珠菌15例(17.4%)、隐球菌12例(13.9%)、马拉色菌10例(11.6%)、酵母菌10例(11.6%)。147例可疑侵袭性真菌感染患者中qRT-PCR检测阳性74例,阳性率50.3%;G试验检测阳性63例,阳性率42.9%;GM试验检测阳性55例,阳性率37.4%。qRT-PCR诊断侵袭性真菌感染灵敏度高于G试验及GM试验,差异具有统计学意义(P<0.05);G实验诊断侵袭性真菌感染特异度高于qRT-PCR及GM试验,但差异无统计学意义(P>0.05)。结论:qRT-PCR对侵袭性真菌感染的检出阳性率高于G试验及GM试验。qRT-PCR诊断侵袭性真菌感染灵敏度高于G试验及GM试验,G实验诊断侵袭性真菌感染特异度高于qRT-PCR及GM试验。Objective To investigate the value of real-time quantitative polymerase chain reaction(qRT-PCR),(1,3)-β-Dglucan test(G test),and galactomannan antigen test(GM test)in the diagnosis of invasive fungal infection.Methods A total of 147 patients with suspected invasive fungal infection who were treated in the First People's Hospital of Zhengzhou from January 2020 to June 2022 were selected as the research subjects,and the fungal culture method was used to identify the fungal type in the infection group.The samples of patients with suspected invasive fungal infection were detected by qRT-PCR,G test and GM test,respectively,and the value of qRT-PCR,G test and GM test in the diagnosis of invasive fungal infection were analyzed with fungal culture results as the standard.Results Among 147 patients with suspected invasive fungal infection,invasive fungi were detected in 86 cases(positive rate 58.5%),including 21 cases of Candida albicans(24.4%),18 cases of Aspergillus(20.9%),and 15 cases of Candida tropicalis(17.4%),12 cases of cryptococcus(13.9%),10 cases of Malassezia(11.6%),and 10 cases of yeast(11.6%).Among the 147 patients with suspected invasive fungal infection,74 cases were positive by qRT-PCR,with a positive rate of 50.3%;63 cases were positive by G test,with a positive rate of 42.9%;55 cases were positive by GM test,with a positive rate of 37.4%.The sensitivity of qRT-PCR in diagnosing invasive fungal infection was higher than that of G test and GM test,and the difference was statistically significant(P<0.05).The specificity of G test for diagnosing invasive fungal infection was higher than that of qRT-PCR and GM test,but the difference was not statistically significant(P>0.05).Conclusion The positive rate of qRT-PCR for invasive fungal infection was higher than that of G test and GM test.The sensitivity of qRT-PCR in diagnosing invasive fungal infection was higher than that of G test and GM test,and the specificity of G test in diagnosing invasive fungal infection was higher than that of qRT-PCR and GM test.

关 键 词：侵袭性真菌感染 实时荧光定量聚合酶链式反应 (1 3)-β-D-葡聚糖试验 半乳甘露聚糖抗原试验 

分 类 号：R725[医药卫生—儿科]