褪黑素对骨质疏松大鼠骨代谢及成骨细胞Wnt/β-catenin信号通路的影响  

Effect of melatonin on bone metabolism and osteoblast Wnt/β-catenin signaling pathway in osteoporotic rats

作　　者：郑俊杰 陈冬冬[1] 林佳生[1] 严伟[1] 洪嘉祥 宋平 陈嵘[1] ZHENG Junjie;CHEN Dongdong;LIN Jiasheng;YAN Wei;HONG Jiaxiang;SONG Ping;CHEN Rong(Department of Bone and Soft Tissue Oncology,Fuzhou Second Hospital,Fuzhou,Fujian 350007,China)

机构地区：[1]福建省福州市第二医院骨与软组织肿瘤科,福州350007

出　　处：《福建医药杂志》2023年第4期111-114,共4页Fujian Medical Journal

基　　金：福建省科技创新平台项目“2020年福建省创伤骨科急救与康复临床医学研究中心”(2020Y2014)。

摘　　要：目的 探讨褪黑素对骨质疏松大鼠骨代谢及成骨细胞Wnt/β-catenin信号通路的影响。方法 按照随机数字表法将60只大鼠分成空白组、模型组和褪黑素组。空白组使用与褪黑素同等剂量的生理盐水进行灌胃;模型组在空白组的基础上行双侧卵巢切除术,构建骨质疏松大鼠模型;褪黑素组在模型组的基础上,采用静脉注射的方式给予褪黑素治疗。采用ELISA法检测3组大鼠血清中碱性磷酸酶(ALP)、Ⅰ型前胶原氨基端原肽(PINP)、骨钙素(OC)、I型胶原C端交联肽(CTX-Ⅰ)的含量。采用RT-PCR及Western blot法检测大鼠成骨细胞中Wnt/β-catenin信号通路相关因子细胞β-连环蛋白(β-catenin)、无翅型MMTV整合位点家族成员3A(Wnt3a)、无翅型MMTV整合位点家族成员7B(Wnt7b)、轴抑制蛋白2抗体(Axin2)的mRNA及蛋白表达。结果 与模型组比较,褪黑素组大鼠血清中的ALP、PINP、OC、CTX-Ⅰ含量得到提升,差异有统计学意义(P<0.05)。与模型组比较,褪黑素组大鼠成骨细胞的β-catenin、Wnt7b的mRNA和蛋白含量明显升高(P<0.05),Axin2的mRNA和蛋白含量明显降低(P<0.05)。结论 褪黑素能够调节骨代谢平衡,并通过激活Wnt/β-catenin信号通路促进成骨细胞的形成。Objective To investigate the effect of melatonin on bone metabolism and osteoblast Wnt/β-catenin signaling pathway in osteoporotic rats.Methods According to the random number table method,60 rats were divided into blank group,model group and melatonin group.The blank group was intragastrically administered with the same dose of normal saline as melatonin;the model group underwent bilateral ovariectomy on the basis of the blank group to construct a rat model of osteoporosis;on the basis of the model group,the melatonin group was treated with melatonin by intravenous injection.The contents of alkaline phosphatase(ALP),type I procollagen amino terminal propeptide(PINP),osteocalcin(OC)and type I collagen C-terminal cross-linked peptide(CTX-I)in serum of rats in the three groups were detected by ELISA.The mRNA and protein expression of Wnt/β-catenin signaling pathway related factors β-catenin,Wnt3a,Wnt7b and Axin2 in rat osteoblasts were detected by RT-PCR and Western blot.Results Compared with the model group,the contents of ALP,PINP,OC and CTX-I in the serum of the melatonin group were increased significantly(P<0.05).Compared with the model group,the mRNA and protein levels of β-catenin and Wnt7b in osteoblasts of rats in the melatonin group were significantly increased(P<0.05),and the mRNA and protein levels of Axin2 were significantly decreased(P<0.05).Conclusion Melatonin can regulate the balance of bone metabolism and promote the formation of osteoblasts by activating the Wnt/β-catenin signaling pathway.

关 键 词：褪黑素 骨质疏松 骨代谢 WNT/Β-CATENIN 

分 类 号：R58[医药卫生—内分泌]