降解多环芳烃和异丙威的耐镉菌种筛选及其特性研究  

Characteristics and Isolation of Cd-Resistant Microorganism Strains Used in the Biodegradation of Polycyclic Aromatic Hydrocarbons and Isoprocarb

作　　者：吴民熙 王震 曹如亮 方雅瑜 黄庆 刘惠琼 尹红梅 杜东霞 高帅帅 许丽娟 WU Min-xi;WANG Zhen;CAO Ru-liang;FANG Ya-yu;HUANG Qing;LIU Hui-qiong;YIN Hong-mei;DU Dong-xia;GAO Shuai-shuai;XU Li-juan(Hunan Institute of Microbiology,Changsha 410009,PRC;Agriculture and Rural Affairs Bureau of Heshan District,Yiyang 413000,PRC;Hunan Hengkai Environmental Protection Technology Investment Co.,Ltd.,Changsha 410221,PRC;Central South Water Science and Technology Co.,Ltd.,Changsha 410009,PRC)

机构地区：[1]湖南省微生物研究院,湖南长沙410009 [2]益阳市赫山区农业农村局,湖南益阳413000 [3]湖南恒凯环保科技投资有限公司,湖南长沙410221 [4]中南水务科技有限公司,湖南长沙410009

出　　处：《湖南农业科学》2023年第7期11-19,共9页Hunan Agricultural Sciences

基　　金：湖南省自然科学基金面上项目(2021JJ30412);长沙市科技局自然科学基金项目(kq2202329,kq2208131);湖南省农业农村厅2021年“一化四体系”农业科研计划(湘农20210001);湖南省微生物研究院课题(2021zy09)。

摘　　要：为获得既耐受重金属镉又能高效降解多环芳烃(菲、芘)、异丙威的菌种,利用选择培养基从染土壤中分离高效降解微生物,并进行菌种分类和降解特性分析。共筛选获得2个耐镉并降解多环芳烃、异丙威的菌种,其中WLX8D对芘降解效率最高;S-200培养15 d内对菲和异丙威的最高降解效率分别为53.6%和66.7%,结合形态学特征和16S rDNA序列分析,初步鉴定结果,WLX8D为意大利农霉菌(Agromyces italicus),S-200为硫酸盐还原菌(Salfate reducing bacteria)。WLX8D、S-200对芘、菲的作用浓度和耐镉浓度范围广,WLX8D可降解25~100 mg/L浓度范围的芘,S-200可以高效降解菲和异丙威(降解率50%~60%),2菌株在镉浓度25~200 mg/L的培养基中生长良好。因此,WLX8D、S-200在修复镉与多环芳烃复合污染土壤方面具有潜在的应用价值。To screen out Cd-resistant microorganisms that can also efficiently degrade polycyclic aromatic hydrocarbons(PAHs:phenanthrene and pyrene) or isoprocarb,selective media were used with PAHs or isoprocarb as the sole carbon source.The phylogenetic position of the obtained strains and their degradation characteristics of PAHs and isoprocarb were analyzed.Finally,two Cd-resistant strains which were capable of degrading PAHs or isoprocarb were isolated from contaminated soil.Among them,strain WLX8D was of the highest degradation efficiency for pyrene;as for strain S-200,within 15 days of culture,its maximum degradation efficiency for phenanthrene and isoprocarb was 53.6% and 66.7%,respectively.Based on morphologic characteristics and a 16S rDNA sequence analysis,strain WLX8D was identified as Agromyces italicus,and strain S-200 was a salfate reducing bacterium.WLX8D and S-200 could efficiently degrade diverse concentrations of PAHs(phenanthrene,pyrene) and isoprocarb over a wide range of Cd concentrations(25-200mg/L).WLX8D was efficient for 25-100 mg/L of pyrene.S-200 reached a 50% degradation rate for phenanthrene and 60% for isoprocarb.These two strains grew well on the culture medium containing 25-200 mg/L of Cd.These results indicate that WLX8D and S-200 are efficient strains for PAHs and isoprocarb biodegradation and have potential in the bioremediation of Cd and PAHs contaminated soil.

关 键 词：耐镉 多环芳烃 异丙威 生物降解 意大利农霉菌 硫酸盐还原菌 

分 类 号：Q935[生物学—微生物学] X53[环境科学与工程—环境工程]