针刺后溪和环跳对腰椎间盘突出症模型大鼠NF-κB/iNOS/NO通路的影响  

Effects of acupuncture at Houxi(SI3)and Huantiao(GB30)on NF-κB/iNOS/NO pathway in lumbar disc herniation model rats

作　　者：楼金成 苏嘉琪 张少巍 胡情 苗镡允 翟春涛 吕玉娥 LOU Jincheng;SU Jiaqi;ZHANG Shaowei;HU Qing;MIAO Xinyun;ZHAI Chuntao;LÜYu’e(Shanxi University of Chinese Medicine,Jinzhong 030600,China;Shanxi Acupuncture Hospital,Taiyuan 030006,China)

机构地区：[1]Shanxi University of Chinese Medicine,Jinzhong 030600,China [2]Shanxi Acupuncture Hospital,Taiyuan 030006,China

出　　处：《Journal of Acupuncture and Tuina Science》2023年第4期254-264,共11页针灸推拿医学（英文版）

基　　金：山西省自然科学基金面上项目,No.201801D121349;山西中医药大学创新团队项目-吕景山针药结合科技创新团队,No.2019TD-003;山西中医药大学创新团队项-吕景山针灸与生物电子医学创新团队,No.2022TD1006;山西省“四个一批”科技兴医创新计划重点攻关专项,No.2022XM35;吕氏脐药灸治疗失眠症患者静息态功能磁共振成像研究,No.201903D321233;山西省科技创新人才团队建设项目,No.202204051002011.

摘　　要：目的:观察针刺后溪和环跳对腰椎间盘突出症(LDH)模型大鼠L5脊神经根核因子κB(NF-κB)炎性信号通路中NF-κB、诱导型一氧化氮合酶(iNOS)及一氧化氮(NO)表达的影响,探讨针刺治疗LDH的作用机制.方法:将40只健康无特定病原体级雄性Sprague-Dawley大鼠按随机数字表法分为假手术组、模型组、针刺1组和针刺2组,每组10只.通过穿刺L4-L5棘突间隙注射自体髓核混悬液制成非压迫髓核突出模型.针刺1组针刺双侧肾俞、大肠俞和委中;针刺2组针刺双侧后溪和环跳.均采用平补平泻手法,每次留针30 min,每隔10 min行针1次,每日1次,共治疗14次.用热刺痛仪进行缩爪痛阈值测定;酶联免疫吸附法测定血清NF-κB、iNOS及NO水平;苏木素-伊红(HE)染色法观察脊神经根的病理形态学改变;定量反转录聚合酶链式反应法检测脊神经根iNOS mRNA表达;免疫荧光法检测脊神经根NF-κB及iNOS蛋白表达.结果:与假手术组比较,模型组的缩爪痛阈值降低;血清NF-κB、iNOS及NO表达水平升高;HE染色观察发现脊神经根内施万细胞大量变性溶解,并出现空泡样改变;同时脊神经根NF-κB、iNOS蛋白表达和iNOS mRNA表达均升高.与模型组比较,针刺1组和针刺2组的缩爪痛阈值升高,针刺2组升高幅度更大(P<0.05);针刺1组和针刺2组的血清NF-κB、iNOS及NO表达水平降低,针刺2组降低更显著(P<0.01);针刺1组和针刺2组的脊神经根空泡样改变和施万细胞变性溶解减少,且针刺2组改变更明显;针刺1组和针刺2组的脊神经根NF-κB及iNOS蛋白表达和iNOS mRNA表达均降低,针刺2组降低更明显(P<0.01).结论:针刺后溪和环跳可改善LDH模型大鼠的脊神经根形态,抑制脊神经根中NF-κB、iNOS蛋白表达和血清NO水平,缓解脊神经根炎症引发的疼痛,这可能是针刺治疗LDH的机制之一.Objective:To observe the effects of acupuncture at Houxi(SI3)and Huantiao(GB30)on the expression levels of nuclear factor kappa B(NF-κB),inducible nitric oxide synthase(iNOS),and nitric oxide(NO)of NF-κB inflammatory signaling pathway in L5 spinal nerve root of lumbar disc herniation(LDH)model rats and explore the mechanism of acupuncture in LDH treatment.Methods:Forty specific-pathogen-free healthy male Sprague-Dawley rats were randomly divided into a sham operation group,a model group,acupuncture group 1,and acupuncture group 2,with 10 rats in each group.The noncompression nucleus protrusion model was made by puncturing L4-L5 spinous process space and injecting autologous nucleus suspension.Acupuncture at bilateral Shenshu(BL23),Dachangshu(BL25),and Weizhong(BL40)was carried out in acupuncture group 1,and acupuncture at bilateral Houxi(SI3)and Huantiao(GB30)in acupuncture group 2.All rats were treated with balanced reinforcing and reducing needling manipulations,and the needles were retained for 30 min/time with one episode of needling manipulation every 10 min,once a day,14 times in total.The threshold value of paw withdrawal pain was measured by a thermal stimulation pain instrument;the serum NF-κB,iNOS,and NO levels were measured by enzyme-linked immunosorbent assay.The pathomorphological changes of spinal nerve roots were observed by hematoxylin-eosin(HE)staining;quantitative reverse transcription polymerase chain reaction was used to detect iNOS mRNA expression in spinal nerve roots;the NF-κB and iNOS protein expression in spinal nerve roots was detected by the immunofluorescence method.Results:Compared with the sham operation group,the threshold of paw withdrawal pain in the model group was decreased,and the expression levels of serum NF-κB,iNOS,and NO were increased;HE staining showed many degenerated and dissolved Schwann cells in spinal nerve roots with vacuolar changes;meanwhile,the expression levels of NF-κB and iNOS proteins,and the iNOS mRNA in spinal nerve roots were increased.Compared with

关 键 词：针刺疗法 穴 后溪 环跳 椎间盘移位 核因子ΚB 诱导型一氧化氮合酶 一氧化氮 大鼠 

分 类 号：R2-03[医药卫生—中医学]