雀啄灸对痛经大鼠施灸穴区及子宫温度、血流灌注和血清AVP、PGF_(2α)及TXB_(2)的影响  

Effects of sparrow-pecking moxibustion on the temperature and blood flow perfusion of moxibustion point area and uterus and serum AVP,PGF_(2α),and TXB_(2)in rats with dysmenorrhea

作　　者：周晨曦 李新华 孟祥云 刘超 张选平 孙艳秋 张璇 梁玉磊 赵志国 ZHOU Chenxi;LI Xinhua;MENG Xiangyun;LIU Chao;ZHANG Xuanping;SUN Yanqiu;ZHANG Xuan;LIANG Yulei;ZHAO Zhiguo(Hebei University of Chinese Medicine,Shijiazhuang 050091,China;Key Research Office of Hebei Provincial Needling and Moxibustion Techniques Effect Specificity,Shijiazhuang 050091,China)

机构地区：[1]Hebei University of Chinese Medicine,Shijiazhuang 050091,China [2]Key Research Office of Hebei Provincial Needling and Moxibustion Techniques Effect Specificity,Shijiazhuang 050091,China

出　　处：《Journal of Acupuncture and Tuina Science》2023年第4期265-272,共8页针灸推拿医学（英文版）

基　　金：河北省高等学校科学技术研究项目,No.ZD2019099。

摘　　要：目的:观察雀啄灸神阙、关元对寒湿凝滞型原发性痛经(PD)大鼠扭体反应及评分,施灸穴区及子宫温度值,子宫血流灌注量,大鼠血清精氨酸血管加压素(AVP),前列腺素(PG)F_(2α)及血栓素(TX)B_(2)含量的影响,探讨雀啄灸治疗PD的可能机制.方法:将32只雌性未孕健康Wistar大鼠采用随机数字表法分为正常组、模型组、布洛芬组及雀啄灸组,每组8只.除正常组外,其余三组采用冷水浴结合苯甲酸雌二醇复合缩宫素注射的方法造模.雀啄灸组大鼠于造模第8天接受雀啄灸神阙和关元治疗,每次30 min,1次/日,连续3 d;布洛芬组大鼠于造模第8天接受布洛芬溶液(规格为10 mL,125 mg)0.8 mL灌服治疗,1次/日,连续3 d;正常组与模型组给予0.8 mL生理盐水灌胃,1次/日,连续3 d.第11天,各组大鼠腹腔注射缩宫素(2 U/只).观察各组大鼠20 min内扭体潜伏期及扭体评分,观测大鼠神阙、关元穴区及在体子宫的温度值及血流灌注量,检测大鼠血清中AVP、PGF_(2α)及TXB_(2)的含量.结果:雀啄灸组及布洛芬组扭体潜伏期较模型组明显延长(P<0.01),扭体评分显著降低(P<0.01);与布洛芬组比较,雀啄灸组扭体潜伏期延长(P<0.01),扭体评分降低(P<0.01);与正常组比较,模型组大鼠神阙、关元穴区及子宫温度值,以及子宫血流灌注量明显降低,血清PGF_(2α)、AVP及TXB_(2)含量明显升高(P<0.01);与模型组比较,布洛芬组、雀啄灸组大鼠神阙、关元穴区及子宫温度值,以及子宫血流灌注量均明显升高,血清PGF_(2α)、AVP及TXB_(2)的含量明显降低(P<0.05或P<0.01);与布洛芬组比较,雀啄灸组大鼠神阙、关元穴区及子宫温度值,以及子宫血流灌注量均明显升高(P<0.05),血清AVP及TXB_(2)含量明显降低(P<0.05),血清PGF_(2α)含量无统计学差异(P>0.05).结论:雀啄灸对寒湿凝滞型原发性痛经镇痛效果显著,其机制可能与提高灸治穴区、子宫温度值及子宫血流灌注量,降低血清PGF_(2αObjective:To observe the effects of sparrow-pecking moxibustion at Shenque(CV8)and Guanyuan(CV4)on the writhing reaction and score,the temperature and blood flow perfusion of moxibustion point area and uterus,the serum levels of arginine vasopressin(AVP),prostaglandin(PG)F_(2α),and thromboxane(TX)B_(2) in rats with primary dysmenorrhea(PD)due to cold-dampness stagnation,and to explore the possible mechanism of sparrow-pecking moxibustion in treating PD.Methods:Thirty-two healthy non-pregnant female Wistar rats were randomly divided into a normal group,a model group,an ibuprofen group,and a sparrow-pecking moxibustion group,with 8 rats in each group.Except for the normal group,the other three groups were subjected to modeling with cold water bath combined with estradiol benzoate and oxytocin injection.Rats in the sparrow-pecking moxibustion group were treated with sparrow-pecking moxibustion at Shenque(CV8)and Guanyuan(CV4)on the 8th day of modeling,30 min/time,once a day for 3 d;those in the ibuprofen group were treated with 0.8 mL ibuprofen solution(a specification of 125 mg in 10 mL)on the 8th day of modeling,once a day for 3 d;those in the normal group and the model group were given 0.8 mL normal saline,once a day for 3 d.On the 11th day,rats in each group were intraperitoneally injected with oxytocin(2 U/rat),and the writhing incubation period and writhing score in 20 min were observed;the temperature and the blood perfusion of Shenque(CV8),Guanyuan(CV4),and uterus in vivo were detected;the serum levels of AVP,PGF_(2α),and TXB_(2)were determined.Results:The writhing incubation period was significantly longer(P<0.01)and the writhing score was significantly lower(P<0.01)in the sparrow-pecking moxibustion group and the ibuprofen group than in the model group;compared with the ibuprofen group,the writhing incubation period was prolonged(P<0.01)and the writhing score was decreased(P<0.01)in the sparrow-pecking moxibustion group;compared with the normal group,the temperature and the blood perfusion of Shenque(CV8

关 键 词：灸法 雀啄灸 寒凝胞宫 痛经 穴 关元 神阙 大鼠 

分 类 号：R2-03[医药卫生—中医学]