HPLC法同时测定黄连上清片中栀子苷、黄芩苷的含量  

Simultaneous Determination of Geniposide and Baicalin in Huanglian Shangqing Tablets by HPLC

作　　者：彭玲娜 孙辉 丁野 李文莉 赵桂铃 PENG Lingna;SUN Hui;DING Ye;LI Wenli;ZHAO Guiling(Hunan Institute of Drug Inspection and Testing,Hunan Engineering and Technology Research Center for Pharmaceutical Quality Evaluation,Changsha Hunan 410001,China)

机构地区：[1]湖南省药品检验检测研究院,湖南省药品质量评价工程技术中心,湖南长沙410001

出　　处：《药品评价》2023年第5期562-565,共4页Drug Evaluation

摘　　要：目的建立HPLC法,同时测定黄连上清片中黄芩苷和栀子苷的含量。方法以十八烷基键合硅胶为填充剂,0.2%磷酸溶液和乙腈为流动相进行梯度洗脱,流速为1.0 mL/min,波长为250 nm,柱温为35℃,进样量为10μL。结果栀子苷在1.971~985.6μg/m L(y=1003708.57x+33870.22,r=1.0000),黄芩苷在8.500~340.0μg/m L(y=1315348.69x+14934.22,r=1.0000)均呈良好的线性关系;平均回收率分别为96.67%、100.30%。结论含量测定方法操作简单,准确耐用,为黄芩、栀子药材在该制剂中的质量控制和标准改进提供了科学依据。Objective To establish a HPLC method for simultaneous determination of geniposide and baicalin in huanglian shangqing tablets.Methods It was tested with C18 column,using 0.2%phosphoric acid and acetonitrile for gradient elution,the flow rate was 1.0 mL/min,the wavelength was 250 nm,column temperature at 35℃and the injection volume was 10μL.Results The test results show that geniposide and baicalin all had a good linear relationship in the range of 1.971~985.6μg/mL(y=1003708.57x+33870.22,r=1.0000)and 8.500~340.0μg/mL(y=1315348.69x+14934.22,r=1.0000),respectively.The average recovery rate(n=6)was 96.67%and 100.30%,respectively.Conclusion The method is simple,accurate and durable,and provide a scientific basis for the quality control and standard improvement of scutellariae radix and gardeniae fructus in this preparation.

关 键 词：高效液相色谱法 黄连上清片 栀子苷 黄芩苷 

分 类 号：R286.0[医药卫生—中药学]