Enhanced production of cytidine 5'-monophosphate using biocatalysis of di-enzymes immobilized on amino-functionalized sepharose  

作　　者：Xiaohong Zhou Wenfeng Zhou Wei Zhuang Chenjie Zhu Hanjie Ying Hongman Zhang 

机构地区：[1]School of Chemistry and Molecular Engineering,Nanjing Tech University,Nanjing 211816,China [2]State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,National Engineering Technique Research Center for Biotechnology,Nanjing Tech University,Nanjing 211816,China

出　　处：《Chinese Journal of Chemical Engineering》2023年第6期40-52,共13页中国化学工程学报（英文版）

基　　金：supported by grants from the National Key Research and Development Program of China(2021YFC2102805,2019YFD1101204);the National Natural Science Foundation of China(21878142,21776132);Key Research and Development Plan of Jiangsu Province(BE2020712);Key Research and Development Plan of Jiangsu Province(BE2019001);Jiangsu Natural Science Fund for Distinguished Young Scholars(BK20190035);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。

摘　　要：Cytidine 5'-monophosphate(5'-CMP)is an essential nucleotide for additives.In this study,enhanced production of 5'-CMP was realized by the transformation of cytidine using co-immobilized di-enzymes,uridine-cytidine kinase(UCK)and acetate kinase(AcK).The immobilization yield of the enzyme had a clear correlation with the surface charges as zeta potential(ξ).Among them,ε-polylysinefunctionalized sepharose(SA-EPL,ξ=9.31 m V)showed high immobilization yield(78.8%),which was4.9-fold than that of nitrilotriacetic acid functionalized sepharose(SA-NTA,ξ=-12.6 m V).The residual activity of affinity co-immobilized enzyme(EPL-Ni/EPL@Ac K-UCK)was higher than 70.6%after recycled 10 times.Thus,this study provides an effective approach for the production of 5'-CMP with the advantages of low adenosine 5'-triphosphate(ATP)consumption,reduced side reactions,and improved reusability by co-immobilized UCK and Ac K on the functionalized Sepharose.

关 键 词：SEPHAROSE Ε-POLYLYSINE Dual-enzyme cascade Cytidine 5'-monophosphate Enzyme immobilization 

分 类 号：O621.251[理学—有机化学]