肾结石患者肾乳头组织中LINC01197及相关因子的表达研究  

Expression of LINC01197 and related factors in renal papillary tissues from patients with kidney stones

作　　者：林谦[1] 倪建华 耿志海 朱江波[1] 章庆华[1] 郑森中[1] LIN Qian;NI Jianhua;GENG Zhihai;ZHU Jiangbo;ZHANG Qinghua;ZHENG Senzhong(Department of Urology,Taizhou First People’s Hospital,Taizhou 318020,Zhejiang,China)

机构地区：[1]台州市第一人民医院泌尿外科,浙江台州318020

出　　处：《中国现代医生》2023年第23期31-34,39,共5页China Modern Doctor

基　　金：台州市社会科技发展计划项目(21ywa46)。

摘　　要：目的 探讨肾结石患者肾乳头组织中LINC01197、miR-516b-5p、SIRT3、FoxO1、Ac-FoxO1的表达特点及相关性。方法 选取2021年3月至2023年3月台州市第一人民医院收治的经皮肾镜碎石术治疗的30例草酸钙肾结石患者作为结石组,选取同期行根治性肾切除术(术后均由病理科诊断为透明细胞癌)的30例肾肿瘤患者作为对照组。结石组于术中直视下活检肾乳头及其周围间质组织,对照组收集远离肿瘤(2cm以上)的正常肾乳头组织样本,经病理学诊断后确诊为正常肾乳头组织。检测、比较两组肾乳头组织内LINC01197、miR-516b-5p、SIRT3、FoxO1、Ac-FoxO1表达水平,分析LINC01197、miR-516b-5p、SIRT3之间的相关性。结果 与对照组相比,结石组的肾乳头组织呈现LINC01197和SIRT3低表达、miR-516b-5p和Ac-FoxO1/FoxO1高表达,差异均有统计学意义(P<0.05)。Spearman相关性分析显示LINC01197与miR-516b-5p呈显著负相关(r=–0.751,P<0.05),LINC01197与SIRT3呈显著正相关(r=0.813,P<0.05),miR-516b-5p与SIRT3呈显著负相关(r=–0.528,P<0.05)。结论 LINC01197在肾结石病理进展中具有潜在价值,其参与调节肾结石的发展可能跟miR-516b-5p、SIRT3、FoxO1有关。Objective To investigate the expression and correlation of LINC01197,miR-516b-5p,SIRT3,FoxO1 and Ac-FoxO1 in renal papillary tissues of patients with kidney stones.Methods A total of 30 patients with calcium oxalate kidney stones treated by percutaneous nephrolithotripsy admitted to the Taizhou First People’s Hospital from March 2021 to March 2023 were selected as the stone group,and a total of 30 patients with renal tumors who underwent radical nephrectomy(all of whom were diagnosed as clear-cell carcinoma by pathology after the operation)during the same period were selected as the control group.In the stone group,the renal papilla and its surrounding mesenchymal tissues were biopsied under intraoperative direct vision,while in the control group,samples of normal renal papilla tissues far away from the tumor(more than 2cm)were collected,and the diagnosis of normal renal papilla tissues was confirmed after pathological diagnosis.The expression levels of LINC01197,miR-516b-5p,SIRT3,FoxO1,and Ac-FoxO1 were detected and compared in the renal papilla tissues of the two groups,and the correlation between LINC01197,miR-516b-5p,and SIRT3 was analyzed.Results Compared with the control group,the renal papilla tissues of the stone group showed low expression of LINC01197 and SIRT3,and high expression of miR-516b-5p and Ac-FoxO1/FoxO1,all of which were statistically significant(P<0.05).Spearman’s correlation analysis showed a significant negative correlation between LINC01197 and miR-516b-5p(r=–0.751,P<0.05),LINC01197 was significantly positively correlated with SIRT3(r=0.813,P<0.05),and miR-516b-5p was significantly negatively correlated with SIRT3(r=–0.528,P<0.05).Conclusion LINC01197 has potential value in the pathological progression of kidney stones,and its participation in regulating the development of kidney stones may be related to miR-516b-5p,SIRT3 and FoxO1.

关 键 词：长链非编码RNA LINC01197 miR-516b-5p FOXO1 肾结石 

分 类 号：R692.4[医药卫生—泌尿科学]