三七白及粉通过Nrf2/Gpx4介导的铁死亡改善脑出血型应激性溃疡大鼠的胃黏膜损伤  

Panax Nnotoginseng Powder Ameliorates Gastric Mucosal Injury via Nrf2/Gpx4-Mediated Iron Death in Rats with Cerebral Hemorrhagic Stress Ulcers

作　　者：安凯 郭沛然 孙玉凤[3] AN Kai(The Second Ward of Rehabilitation Medicine,Shijiazhuang People's Hospital,Hebei Shijiazhuang 050000,China)

机构地区：[1]河北省石家庄市人民医院康复医学二病区,河北石家庄050000 [2]河北医科大学中西医结合学院,河北石家庄050000 [3]河北医科大学第二医院消化内科,河北石家庄050000

出　　处：《河北医学》2023年第8期1267-1274,共8页Hebei Medicine

基　　金：河北省中医药管理局项目,(编号:2019454)。

摘　　要：目的:探究三七白及粉通过Nrf2/Gpx4介导的铁死亡对脑出血型应激性溃疡大鼠胃黏膜损伤的保护作用。方法:按照随机数字表法将60只大鼠分为NC组、SGU组、三七白及粉低剂量组(三七白及粉L组)、三七白及粉中剂量组(三七白及粉M组)、三七白及粉高剂量组(三七白及粉H组)、三七白及粉高剂量+Nrf2特异性抑制剂ML385组(三七白及粉H+ML385组),每组10只。对大鼠进行神经功能评分;测定胃黏膜溃疡指数;HE染色检测胃组织病理学变化;检测胃组织中Fe^(2+)、MDA含量及SOD活性;DHE荧光染色法检测胃组织中ROS水平;蛋白质印迹检测胃黏膜组织中Nrf2、Gpx4和SLC7A11蛋白表达。结果:和NC组相比,SGU组大鼠神经功能评分、胃黏膜溃疡指数、Fe^(2+)、MDA含量及ROS水平增加,SOD活性降低(P<0.05);和SGU组相比,三七白及粉L、M、H组大鼠神经功能评分、胃黏膜溃疡指数、Fe^(2+)、MDA含量及ROS水平明显降低,SOD活性升高(P<0.05);和三七白及粉H组相比,三七白及粉H+ML385组大鼠神经功能评分、胃黏膜溃疡指数、Fe^(2+)、MDA含量及ROS水平明显升高,SOD活性降低(P<0.05)。和NC组相比,SGU组大鼠胃黏膜组织中Nrf2、Gpx4和SLC7A11蛋白表达明显降低(P<0.05);和SGU组相比,三七白及粉L、M、H组大鼠胃黏膜组织中Nrf2、Gpx4和SLC7A11蛋白表达均明显增加,且呈剂量依赖(P<0.05);三七白及粉H+ML385组大鼠胃黏膜组织中Nrf2、Gpx4和SLC7A11蛋白表达明显低于三七白及粉H组(P<0.05)。结论:三七白及粉对脑出血型应激性溃疡大鼠胃黏膜损伤发挥保护作用,其机制和激活Nrf2/Gpx4通路抑制铁死亡相关。Objective:To investigate the protective effect of Panax notoginseng powder on gastric mucosal injury in rats with cerebral hemorrhagic stress ulcers via Nrf2/Gpx4-mediated iron death.Methods:A total of 60 rats were divided into NC group,SGU group,Panax notoginseng powder low dose group(Panax notoginseng powder L group),Panax notoginseng powder medium dose group(Panax notoginseng powder M group),Panax notoginseng powder high dose group(Panax notoginseng powder H group),Panax notoginseng powder high dose+Nrf2 specific inhibitor ML385 group(Panax notoginseng powder H+ML385 group)according to the random number table method,10 rats in each group.The rats were scored for neurological function;the ulcer index of gastric mucosa was measured;HE staining was performed to detect histopathological changes in gastric tissues;Fe^(2+),MDA content and SOD activity were detected in gastric tissues;ROS levels in gastric tissues were detected by DHE fluorescence staining;Nrf2,Gpx4 and SLC7A11 protein expression in gastric mucosa tissues were detected by protein blotting.Results:Compared with the NC group,the neurological function score,gastric mucosal ulcer index,Fe^(2+),MDA content and ROS level increased and SOD activity decreased in the SGU group(P<0.05);compared with the SGU group,the neurological function score,gastric mucosal ulcer index,Fe^(2+),MDA content and ROS level decreased significantly and SOD activity increased in the Panax ginseng powder L,M and H groups(P<0.05);compared with the Panax ginseng powder H group,the neurological function score,gastric mucosal ulcer index,Fe^(2+),MDA content and ROS level of rats in the Panax ginseng powder H+ML385 group were significantly higher and SOD activity was lower(P<0.05).Compared with the NC group,Nrf2,Gpx4 and SLC7A11 protein expression in the gastric mucosa tissues of rats in the SGU group were significantly decreased(P<0.05);compared with the SGU group,Nrf2,Gpx4 and SLC7A11 protein expression in the gastric mucosa tissues of rats in the Panax ginseng powder L,M and H groups

关 键 词：三七白及粉 Nrf2/Gpx4 铁死亡 脑出血型应激性溃疡 胃黏膜损伤 

分 类 号：R285.5[医药卫生—中药学]