蛭龙活血通瘀胶囊与氯吡格雷联用对阿司匹林抵抗大鼠的疗效及机制研究  

Study on Therapeutic Effect and Mechanism of Zhilong Huoxue Tongyu Capsule Combined with Clopidogrel on Aspirin Resistance in Rats

作　　者：邓谷霖 黄新武 许红 李秋月 李国春 DENG Gulin;HUANG Xinwu;XU Hong;LI Qiuyue;LI Guochun(The Affiliated Hospital of Traditional Chinese Medicine,Southwest Medical University,Luzhou 646000,China;School of Pharmacy,Southwest Medical University,Luzhou 646003,China;National Traditional Chinese Medicine Clinical Research Base,Luzhou 646000,China)

机构地区：[1]西南医科大学附属中医医院,四川泸州646000 [2]西南医科大学药学院,四川泸州646003 [3]国家中医临床研究基地建设单位,四川泸州646000

出　　处：《中国中医基础医学杂志》2023年第8期1284-1287,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家中医临床研究基地建设单位科研项目(西南医大中医院【2020】33号)。

摘　　要：目的观察蛭龙活血通瘀胶囊与氯吡格雷联用对阿司匹林抵抗模型大鼠的疗效,并探索其作用机制。方法采用高脂高糖高盐饲料加乙酰水杨酸、布洛芬灌胃造模,12周后将造模成功的40只大鼠随机分为模型组、蛭龙胶囊组、氯吡格雷组和联合用药组,每组10只,各组大鼠分别以相应药物灌胃。另取10只大鼠作为正常对照组。给药4周后,各组大鼠采用血栓弹力图(thromboela-stogram,TEG)法检测血小板聚集率,ELISA法检测血栓素(thromboxane B2,TX)B2和6-酮-前列腺素(6-ketoprostaglandin,6-keto-PGF)F1α水平,荧光定量PCR法检测mi RNA-126-3p表达水平。结果与正常组比较,模型组大鼠TEG最大幅度和TXB2水平上升(P<0.05),6-keto-PGF1α和mi RNA-126-3p表达水平下降(P<0.05)。与模型组比较,各用药组大鼠TEG最大幅度和TXB2水平降低(P<0.05),6-keto-PGF1α和mi RNA-126-3p表达水平升高(P<0.05),其中联合用药效果最明显。结论蛭龙活血通瘀胶囊与氯吡格雷联合用药能降低阿司匹林抵抗大鼠血小板聚集率,且比单独使用蛭龙活血通瘀胶囊和氯吡格雷效果更好,其机制可能与调控mi RNA-126-3p的表达水平有关。Objective To observe the effect of Zhilong Huoxue Tongyu capsule combined with clopidogrel on aspirin resistance model rats and explore its mechanism.Methods The model was established by gavage with high-fat,high sugar and high salt diet plus acetylsalicylic acid and ibuprofen.After 12 weeks,40 rats were randomly divided into model group,Zhilong capsule group,clopidogrel group and combined drug group,with 10 rats in each group.Each group was gavaged with corresponding drugs.Another 10 rats were taken as the normal control group.After 4 weeks of administration,the platelet aggregation rate of rats in each group was detected by thromboela-stogram(TEG)method,and thromboxane(TX)B2 and 6-keto-prostaglandin(6-keto-PG)F1αwere detected by ELISA.The expression level of miRNA-126-3p was detected by fluorescence quantitative PCR.Results Compared with the normal group,the maximum amplitude of TEG and TXB2 increased significantly(P<0.05),6-keto-PGF1αand miRNA-126-3p decreased significantly(P<0.05)in rats of model group.Compared with the model group,the maximum amplitude of TEG and TXB2 decreased,while 6-keto-PGF1αand miRNA-126-3p increased(P<0.05)in rats of each treatment group(P<0.05),and the effect of the combination treatment was the best.Conclusion The combination of Zhilong Huoxue Tongyu capsule and clopidogrel can reduce the platelet aggregation rate of aspirin resistant rats,and the combination treatment showed a superior effect compared to the use of Zhilong Huoxue Tongyu capsule or clopidogrel alone.The mechanism may be related to the regulation of the expression level of miRNA-126-3p.

关 键 词：蛭龙活血通瘀胶囊 氯吡格雷 阿司匹林抵抗 血小板聚集功能 miRNA-126-3p 

分 类 号：R285.5[医药卫生—中药学]