全三七片对血管内皮细胞损伤的保护作用研究  

Protective Effect of QuanSanqi Tablets on Vascular Endothelial Cell Injury

作　　者：郭琰 杨庆[3] 李琦[3] 陈颖[3] 蔡维艳[3] 翁小刚[3] 吴德松 崔涛 朱兆云 朱晓新[3] GUO Yan;YANG Qing;LI Qi;CHEN Ying;CAI Weiyan;WENG Xiaogang;WU Desong;CUI Tao;ZHU Zhaoyun;ZHU Xiaoxin(Yunnan Institute of Materia Medica,Kunming 650111,China;Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation,Kunming 650111,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]云南省药物研究所,昆明650111 [2]云南省中药和民族药新药创制企业重点实验室,昆明650111 [3]中国中医科学院中药研究所,北京100700

出　　处：《中国中医基础医学杂志》2023年第8期1352-1357,共6页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：云南省重大科技专项(2019ZF016);云南省重点研发计划项目(202203AC100008)。

摘　　要：目的观察全三七片(PSQ)对炎症和脂质过氧化引起的血管内皮损伤的保护作用。方法以人脐静脉内皮细胞(ECV304)为研究对象,分别采用炎症介质肿瘤坏死因子(tumor necrosis factor,TNF)-α和氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)诱导ECV304血管内皮细胞建立损伤模型,设正常对照组、模型对照组、阳性药组和PSQ不同浓度组,检测血管内皮功能指标一氧化氮(nitric oxide,NO)、总一氧化氮合成酶(total nitric oxide synthase,T-NOS)和内皮素(endothelin,ET)-1,氧化应激相关指标丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)和炎症相关指标白介素(interleukin,IL)-1β、IL-6和TNF-α。结果TNF-α造模24 h后,与正常对照组比较,模型对照组细胞上清中的ET-1、NO、MDA、IL-1β和IL-6水平显著升高(P<0.05或P<0.01),SOD水平显著降低(P<0.05);与模型对照组比较,PSQ不同浓度组细胞上清中的ET-1、NO、MDA、IL-1β和IL-6水平显著降低(P<0.05或P<0.01),SOD水平显著升高(P<0.01)。ox-LDL造模24 h后,与正常对照组比较,模型对照组细胞上清中的ET-1、MDA、IL-1β和TNF-α水平显著升高(P<0.01);与模型对照组比较,PSQ不同浓度组细胞上清中的NO、MDA、T-NOS、ET-1、IL-1β和TNF-α水平显著降低(P<0.05或P<0.01),SOD水平显著升高(P<0.05或P<0.01)。结论全三七片可能通过改善氧化应激反应、保护血管内皮功能以及减少炎症因子的分泌进而保护炎症和脂质过氧化诱导的血管内皮损伤。Objective To observe the protective effect of QuanSanqi tablets(PSQ)on vascular endothelial injury caused by inflammation and lipid peroxidation.Methods Human umbilical vein endothelial cells(ECV304)were used as the study object,and the injury model of ECV304 vascular endothelial cells was established by the inflammatory mediators'tumor necrosis factor(TNF)-αand oxidized low density lipoprotein(ox-LDL),respectively.Normal control group,model control group,positive drug group,and PSQ groups with different concentrations were set up.Nitric oxide(NO),total nitric oxide synthase(T-NOS)and endothelin(ET)-1,malondialdehyde(MDA)and superoxide dismutase(SOD),and interleukin(IL)-1β,IL-6 and TNF-αwere detected.Results After 24 hours of TNF-αmodeling,compared with the normal group,the levels of ET-1,NO,MDA,IL-1βand IL-6 in the supernatant of the model group were significantly increased(P<0.05 or P<0.01),and the level of SOD was significantly decreased(P<0.05).Compared with the model group,PSQ could significantly decrease the levels of ET-1,NO,MDA,IL-1β,and IL-6 in the supernatant(P<0.05 or P<0.01),and increased the level of SOD(P<0.01).After 24 h of ox-LDL modeling,the levels of ET-1,MDA,IL-1β,and TNF-αin the supernatant of the model group were significantly increased compared with the normal group(P<0.01).Compared with the model group,PSQ significantly decreased the levels of NO,TNOS,ET-1,MDA,IL-1β,and TNF-αin the supernatant(P<0.05 or P<0.01),and increased the level of SOD(P<0.05 or P<0.01).Conclusion QuanSanqi tablets may protect against vascular endothelial injury by improving oxidative stress response,protecting vascular endothelial function,and reducing the secretion of inflammatory factors.

关 键 词：全三七片 血管内皮功能 氧化应激 炎症介质 

分 类 号：R285[医药卫生—中药学]