木本植物再生和遗传转化的研究进展  

Research Progress on Regeneration and Genetic Transformation of Woody Plants

作　　者：胡欣荃 古今 杨姝琦 彭昌操 Hu Xinquan;Gu Jin;Yang Shuqi;Peng Changcao(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,510642)

机构地区：[1]华南农业大学林学与风景园林学院,广州510642

出　　处：《分子植物育种》2023年第16期5322-5329,共8页Molecular Plant Breeding

基　　金：广东省林业科技创新重点项目(2016KJCX003,2019KJCX001);国家重点研发计划项目(2016YFD0600104);国家自然科学基金项目(30771759,31170636,31470681);广东省自然科学基金项目(2016A030311032);广州市科技计划项目(201607020024);广东省高等教育青年创新人才基金项目(2017KQNCX017)共同资助。

摘　　要：木本植物作为重要的自然资源,因其生长周期长、器官分化程度较高的特点,使得林木传统育种发展缓慢。进行植物的遗传转化已成为推动林木基因工程发展的重要工具,转基因植株的获得对于植物功能基因的研究有着非常重要的作用。而建立一个稳定的植物遗传转化体系在一定程度上依赖于植物的高效再生。本研究整理了木本植物组织再生和农杆菌介导遗传转化体系构建的相关研究,总结了遇到的困难并提出相应对策。此外,还对林木基因工程的未来发展趋势进行展望,以期为林木遗传改良研究提供参考。Woody plants are important natural resources.However,their long growth cycles and high degree of organ differentiation had slowed the development of traditional tree breeding.The genetic transformation of plants becomes an important tool for the development of forest genetic engineering.Obtaining transgenic plants plays an important part while studying the function of genes.The effectiveness and stability of the genetic transformation system depends on the effectiveness of plant regeneration system to a certain extent.This study reviews the related research on woody plant regeneration and A grobacterium-mediated genetic transformation system,summarizes the difficulties encountered and proposes corresponding countermeasures.In addition,the future development trend of forest genetic engineering is prospected,in order to provide references for other research.

关 键 词：木本植物 再生体系 农杆菌转化法 

分 类 号：S722.3[农业科学—林木遗传育种]