辣椒番茄斑萎病毒(TSWV)分子鉴定及环介导等温扩增(LAMP)检测技术  

Molecular Identification of Tomato Sotted Wilt Virus and Detection of Loop-Mediated Isothermal Amplification Technology in Pepper

作　　者：韦建明 黄鑫 耿坤 李文彪 冯文卓 李云洲 WEI Jianming;HUANG Xin;GENG Kun;LI Wenbiao;FENG Wenzhuo;LI Yunzhou(Department of Plant Pathology,College of Agiculture,Guizhou University,Guiyang 550025,Guizhou,China;Vegetable Technology Promotion Station of Guiyang City,Guizhou Province,Guiyang 550081,Guizhou,China;Bureau of Agriculture and Rural Affairs of Guiyang City,Guiyang 550081,Guizhou,China)

机构地区：[1]贵州大学农学院植物病理教研室,贵州贵阳550025 [2]贵州省贵阳市蔬菜技术推广站,贵州贵阳550081 [3]贵阳市农业农村局,贵州贵阳550081

出　　处：《中国蔬菜》2023年第8期69-78,共10页China Vegetables

基　　金：贵州省科技计划项目(黔科合基础-ZK[2022]一般071);国家自然科学基金项目(32060679);贵州大学培育项目(贵大培育[2019]52号)。

摘　　要：为明确侵染贵州辣椒的番茄斑萎病毒(Tomato spotted wilt virus,TSWV)株系,并建立TSWV环介导等温扩增(LAMP)等快速检测技术,从贵州省辣椒主产区采集疑似感染TSWV的辣椒样本12份和西花蓟马样本10份,并应用RT-PCR、LAMP检测技术,以及基于TSWV-N基因系统发育树构建进行研究。通过分子诊断和测序结果分析,确认疑似感染辣椒样本被TSWV侵染。此外,贵州息烽地区辣椒TSWV病毒病原分离物与贵州番茄TSWV-N分离物相似度最高,为99%。基于这些发现,成功建立了针对辣椒作物和传播媒介西花蓟马的TSWV快速检测系统,为贵州辣椒病毒病害的监测和防控提供有力支持。This study aimed to identify strains of the tomato spotted wilt virus(TSWV)infecting peppers in Guizhou,and establish rapid detection techniques,specifically TSWV loop-mediated isothermal amplification(LAMP).We collected Twelve pepper samples suspected of TSWV infection and ten samples of Western flower thrips from major pepper-producing regions in Guizhou Province.These samples were analyzed using RT-PCR,LAMP detection techniques and hylogenetic tree construction based on TSWV-N genes.Molecular diagnosis and sequencing results confirmed the presence of TSWV in the suspected pepper samples.Additionally,he pathogenic isolates of TSWV in peppers from the Xifeng area of Guizhou exhibited a striking 99%similarity to Guizhou tomato TSWV-N isolates.Building upon these findings,a TSWV rapid detection system for pepper crops and the vector Thrips occidentalis was successfully established,providing strong support for the monitoring and control of pepper virus diseases in Guizhou.

关 键 词：番茄斑萎病毒 西花蓟马 LAMP检测 分子诊断 

分 类 号：S436.418.12[农业科学—农业昆虫与害虫防治]