重金属镉抗性基因czcA原核载体构建与鉴定  

Prokaryotic Vector Construction and Identification of Cadmium Resistance Gene

作　　者：窦敏娜[1] DOU Minna(Xianyang Vocational and Technical College,Xianyang,Shaanxi 712000,China)

机构地区：[1]咸阳职业技术学院,陕西咸阳712000

出　　处：《陕西农业科学》2023年第7期8-10,22,共4页Shaanxi Journal of Agricultural Sciences

摘　　要：首先,将含有强抗镉czcA基因的重组质粒pGM-T载体进行PCR扩增和鉴定,其次,将带有双酶切位点的czcA基因与pGEX-4T-2载体构建,转化于E.coli BL21 DE3中,最后,经PCR鉴定和测序检测均表明试验成功,为下一步czcA基因片段进行诱导融合蛋白的表达提供了前提。In this study,we aimed to construct a prokaryotic vector for the expression of the Cadmium Resistance Gene(czcA).CzcA was amplified from the plasmid of pGM-T by PCR,and subsequently inserted into pGEX-4T-2 vector using a restriction enzyme.By agrose gel electrophoresis and DNA sequencing,czcA gene was induced into pGEX-4T-2 successfully.

关 键 词：镉 czcA pGEX-4T-2 构建 

分 类 号：X531[环境科学与工程—环境工程]