氢气处理促进地黄生长发育及主要药用成分积累  

Hydrogen Gas Treatment Promotes the Growth,Development and the Accumulation of the Main Medicinal Components in Rehmannia glutinosa

作　　者：苏久厂 王一婷 詹娜 胡月然 段红英 SU Jiu-Chang;WANG Yi-Ting;ZHAN Na;HU Yue-Ran;DUAN Hong-Ying(Laboratory of Medicinal Plant Resources and Stress Biology,Department of Bioengineering,College of Life Sciences,Henan Normal University,Xinxiang 453007,Henan,China)

机构地区：[1]河南师范大学生命科学学院生物工程系药用植物资源与逆境生物学研究室,河南新乡453007

出　　处：《中国生物化学与分子生物学报》2023年第7期961-971,共11页Chinese Journal of Biochemistry and Molecular Biology

基　　金：河南省自然科学基金资助项目(No.232300421262);中国博士后科学基金资助项目(No.2022M721053);河南省博士后科研项目(No.202102068);国家自然科学基金项目(No.31870312);河南省高校科技创新团队支持计划项目(No.23IRTSTHN022);河南省科技研发计划联合基金(No.222301420097)资助。

摘　　要：氢气(hydrogen gas,H_(2))在提高农作物品质和产量方面发挥多样且积极的作用。由于栽培方式和化肥农药使用的不当,严重影响了地黄产量和品质。本研究以“金九”地黄为材料,探究氢纳米气泡水(hydrogen nanobubble water,HNW)对不同时期地黄生长发育及重要药用成分积累的影响。与对照组相比,HNW处理7 d后块根不定根数量和发芽率分别提升约217.59%和87.65%。与对照组相比,HNW处理60、75和90 d后,植株叶片冠幅分别增加约42.73%、53.02%和29.42%,最大叶面积分别提高约15.51%、19.74%和11.44%。HNW处理75和90 d后膨大根数较对照组分别增加约105.65%和66.82%。定量PCR分析环烯醚萜苷合成通路关键酶(1-deoxyxylulose-5-phosphate synthetase,DXS;geraniol 10-hydroxylase,G10H;1-deoxy-D-xylulose-5-phosphate reductase,DXR;8-hydroxygeraniol dehydrogenase,10HGO)基因表达发现,HNW处理75 d后RgDXS2、RgG10H 1和RgDXR 1表达量分别约是对照组的2.61、1.27和3.32倍,HNW处理90 d后RgDXS2、RgG10H 1和Rg10HGO 1表达量分别约是对照组的4.11、1.24和1.77倍。高效液相色谱分析表明,HNW处理75和90 d后梓醇含量较对照组分别增加约103.96%和24.94%,HNW处理75 d后地黄苷D含量提高约33.14%。此外,HNW处理120 d后膨大根数较对照组显著增加,同时RgDXR1、RgG10H 1和Rg10HGO 1表达量分别约是对照组的2.00、1.32和1.36倍。综上所述,HNW处理不仅可以促进地黄生长发育,还可以通过调控环烯醚萜苷合成通路关键酶基因,从而提高其重要药用成分积累。Hydrogen gas(H_(2))plays the diverse and positive roles for improving crop yield and quality.Due to the influences of cultivated methods,chemical fertilizers and pesticides,the yield and quality of R.glutinosa have been seriously restricted.Herein,“Jinjiu”R.glutinosa was used as the material to explore the effects of hydrogen nanobubble water(HNW)on the growth,development and the accumulation of the main medicinal components at different stages.Compared with controls,the number of adventitious roots and the germination rate were increased by about 217.59%and 87.65%,respectively,after 7 days(d)of HNW treatment.Compared to controls,after HNW treatment for 60,75 and 90 d,the leaf crown breadth had increased by approximately 42.73%,53.02%and 29.42%,respectively.And the maximum leaf area was elevated by approximately 15.51%,19.74%and 11.44%.After 75 and 90 d with HNW supplement,the number of enlarged roots was increased by about 105.65%and 66.82%than controls,respectively.The analysis of gene expression of iridoid glycoside synthesis pathway enzyme(1-deoxyxylulose-5-phosphate synthetase,DXS;geraniol 10-hydroxylase,G10H;1-deoxy-D-xylulose-5-phosphate reductase,DXR;8-hydroxygeraniol dehydrogenase,10HGO)by using real-time quantitative PCR confirmed that the transcript levels of RgDXS2,RgG10H 1 and RgDXR 1 were significantly upregulated(about 2.61-,1.27-and 3.32-fold),after being treated with HNW for 75 d.After 90 d of HNW treatment,the expression levels of RgDXS2,RgG10H 1 and Rg10HGO 1 were approximately 4.11-,1.24-and 1.77-fold higher than controls.The analysis for medicinal components by high-performance liquid chromatography showed that,compared to controls,after 75 and 90 d of HNW treatment,the catalpol content was increased by roughly 103.96%and 24.94%,respectively.And the rehmannin D content was elevated by 33.14%after 75 d of HNW treatment.In addition,treatment with HNW significantly increased the number of enlarged roots at 120 d,while the transcript levels of RgDXR1,RgG10H 1 and Rg10HGO 1 were increased by a

关 键 词：氢气 地黄 生长发育 药用成分 

分 类 号：Q945.3[生物学—植物学] S567[农业科学—中草药栽培]