胎盘间充质干细胞促进大鼠急性皮肤创面修复  

Placenta-derived mesenchymal stem cells in promoting acute skin wound healing in rats

作　　者：董鸿斐 黄茜 李先慧 张彦标 王旭阳 王冰 孙红玉 Dong Hongfei;Huang Xi;Li Xianhui;Zhang Yanbiao;Wang Xuyang;Wang Bing;Sun Hongyu(Department of Burns and Plastic Surgery,General Hospital of Western Theater Command,Chengdu 610083,Sichuan Province,China;Department of School of Clinical Medicine,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of Basic Center Laboratory,General Hospital of Western Theater Command,Chengdu 610083,Sichuan Province,China;Sichuan Provincial Key Laboratory of Pancreatic Injury and Repair,Chengdu 610083,Sichuan Province,China)

机构地区：[1]西部战区总医院烧伤整形科,四川省成都市610083 [2]西南医科大学临床医学院,四川省泸州市646000 [3]西部战区总医院基础实验室,四川省成都市610083 [4]胰腺损伤与修复四川省重点实验室,四川省成都市610083

出　　处：《中国组织工程研究》2024年第13期2047-2053,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目(81772001),项目负责人:孙红玉;腹部创伤分级救治及器官功能修复系列研究(2019LH04),项目负责人:孙红玉;西部战区总医院院管课题孵化项目(2021-XZYG-C36),项目负责人:黄茜~~。

摘　　要：背景:目前已有多种间充质干细胞被证实具有促进创面修复的作用,但胎盘间充质干细胞是否可促进急性皮肤创面愈合目前尚缺乏相关研究。目的:探讨胎盘间充质干细胞移植对大鼠急性皮肤损伤愈合的影响。方法:20只SD大鼠采用随机数字表法分为PBS组、干细胞组,每组10只。所有大鼠建立全层皮肤缺损模型,PBS组、干细胞组于建模后即刻和第8天在创面及创缘分别注射PBS、胎盘间充质干细胞。建模后即刻及2,4,6,8,10,12,14 d观察创面愈合情况,在14 d时取大鼠创面处皮肤组织,进行苏木精-伊红染色、Masson染色、免疫组织化学染色及免疫荧光染色。结果与结论:①各组大鼠创面均随治疗时间延长而缩小,干细胞组14 d时创面愈合率、创面上皮化率高于PBS组(P<0.01),创面挛缩比率低于PBS组(P<0.01);②苏木精-伊红染色结果显示,干细胞组皮肤创面修复优于PBS组,创面上皮化程度更高,胶原纤维形态更接近正常皮肤;③Masson染色结果显示,与PBS组相比,干细胞组皮肤创面组织中胶原纤维明显增多且粗大,新生组织内胶原纤维含量更多(P<0.01);④免疫组化染色显示,干细胞组新生毛细血管数量多于PBS组(P<0.01),而肿瘤坏死因子α与白细胞介素6的表达低于PBS组(P<0.01);⑤免疫荧光染色显示,干细胞组新生创面内的M2型巨噬细胞数多于PBS组(P<0.01),M1型巨噬细胞数少于PBS组(P<0.01)。结果表明,胎盘间充质干细胞可加速皮肤创面修复、促进创面上皮化、减轻创面挛缩,可能与促进创面内毛细血管新生、调节胶原纤维生成、抑制炎症、调控巨噬细胞向M2型极化有关。BACKGROUND:Currently,a variety of mesenchymal stem cells have been confirmed to have the effect of promoting wound repair,but there is still a lack of relevant research on whether placenta-derived mesenchymal stem cells can promote acute skin wound healing.OBJECTIVE:To investigate the effect of placenta-derived mesenchymal stem cell transplantation on the healing of acute skin wound in rats.METHODS:Twenty SD rats were divided into PBS group and stem cell group by the random number table method,with 10 rats in each group.All rats were selected to establish a full-thickness skin defect model.In the PBS group and stem cell group,PBS buffer and placenta-derived mesenchymal stem cells were immediately injected on the wound surface and wound margin immediately and on day 8 after modeling.The wound healing was observed immediately and on days 2,4,6,8,10,12,and 14 after modeling.The skin tissue of the wound surface was taken on day 14 and treated with hematoxylin-eosin staining,Masson staining,immunohistochemical staining and immunofluorescence staining.RESULTS AND CONCLUSION:(1)The wound surface of the rats in each group decreased with the prolongation of treatment time.The wound healing rate and wound epithelization rate of the stem cell group at 14 days were higher than those of the PBS group(P<0.01),and the wound contracture rate was lower than that of the PBS group(P<0.01).(2)The results of hematoxylin-eosin staining showed that the skin wound healing of the stem cell group was better than that of the PBS group;the degree of wound epithelization was higher,and the morphology of collagen fibers was close to that of normal skin.(3)Masson staining results showed that compared with the PBS group,collagen fibers in the skin wound tissue of the stem cell group were significantly increased and thicker,and the content of collagen fibers in the new tissue was significantly higher than that of the PBS group(P<0.01).(4)Immunohistochemical staining showed that the number of new capillaries in the stem cell group was higher than

关 键 词：间充质干细胞 胎盘 创面修复 巨噬细胞极化 皮肤 血管新生 

分 类 号：R459.9[医药卫生—治疗学] R318.19[医药卫生—临床医学] R641