有氧运动对阿尔茨海默症小鼠成年海马神经发生的影响  

Effects of aerobic exercise on adult hippocampal neurogenesis in Alzheimer’s disease mice

作　　者：张业廷 李垂坤[2] 魏翠兰[3] 付燕 张飞飞 Zhang Yeting;Li Chuikun;Wei Cuilan;Fu Yan;Zhang Feifei(Civil Aviation Flight University of China,Guanghan 618307,Sichuan Province,China;College of Physical Education,Chengdu University,Chengdu 610106,Sichuan Province,China;Sports Institute of Chengdu University of Technology,Chengdu 610059,Sichuan Province,China;College of Physical Education,Southwest Minzu University,Chengdu 610041,Sichuan Province,China)

机构地区：[1]中国民用航空飞行学院,四川省广汉市618307 [2]成都大学体育学院,四川省成都市610106 [3]成都理工大学体育学院,四川省成都市610059 [4]西南民族大学体育学院,四川省成都市610041

出　　处：《中国组织工程研究》2024年第13期2068-2075,共8页Chinese Journal of Tissue Engineering Research

基　　金：四川省科技计划项目(2020YFH0184),项目负责人:张业廷;中央高校基本科研业务费专项资金资助(PHD2023-003),项目负责人:张业廷~~。

摘　　要：背景:运动有助于改善阿尔茨海默症、痴呆症以及与年龄相关的认知能力,运动与这些健康益处之间的一个潜在中介可能是成年海马神经发生。因此,探索运动如何影响阿尔茨海默症小鼠的成年海马神经发生过程具有重要意义。目的:观察有氧运动对阿尔茨海默症小鼠成年海马神经发生的影响,探究有氧运动能否促进其成年海马神经发生。方法:将3月龄野生型(C57BL/6Jnju)及APP/PS1双转基因阿尔茨海默症小鼠随机分为4组:野生对照组、野生运动组、阿尔茨海默症对照组、阿尔茨海默症运动组,每组20只。对照组小鼠不进行运动,运动组小鼠进行5个月的有氧运动干预。运动干预结束后,采用Real-time PCR、免疫荧光及Western blot检测各组小鼠海马组织DCX、Ki67、βⅢ-tubulin及NeuN的表达。结果与结论:阿尔茨海默症对照组小鼠海马齿状回区DCX、βⅢ-tubulin及NeuN表达均显著低于野生对照组小鼠(P<0.05);阿尔茨海默症运动组小鼠海马齿状回区DCX、Ki67、βⅢ-tubulin及NeuN表达显著高于阿尔茨海默症对照组(P<0.05)。提示:长期有氧运动干预后,可以促进阿尔茨海默症小鼠成年海马神经发生过程中神经元增殖、迁移分化,显著增加神经元前体细胞、新生神经元数量。BACKGROUND:Exercise improves Alzheimer’s disease,dementia,and age-related cognitive abilities.A potential mediator between exercise and these health benefits may be adult hippocampal neurogenesis.Therefore,it is of great significance to explore whether and how exercise affects the adult hippocampal neurogenesis process in Alzheimer’s disease mice.OBJECTIVE:To observe the effect of aerobic exercise on adult hippocampal neurogenesis of Alzheimer’s disease mice,and to explore whether aerobic exercise can promote their adult hippocampal neurogenesis.METHODS:Three-month-old wild-type(C57BL/6Jnju)and APP/PS1 double transgenic Alzheimer’s disease mice were randomly divided into four groups:wild control group,wild exercise group,Alzheimer’s disease control group and Alzheimer’s disease exercise group,with 20 mice in each group.The control group did not do exercise,and the exercise group did aerobic exercise for 5 months.After exercise intervention,real-time PCR,immunofluorescence and western blot assay were used to detect the expression levels of DCX,Ki67,βIII-tubulin and NeuN in the hippocampal tissue of mice in each group.RESULTS AND CONCLUSION:The expressions of DCX,βIII-tubulin and NeuN in the hippocampal dentate gyrus in the Alzheimer’s disease control group were significantly lower than those in the wild control group(P<0.05).The expressions of DCX,Ki67,βIII-tubulin and NeuN were significantly higher in the hippocampal dentate gyrus in the Alzheimer’s disease exercise group than those in the Alzheimer’s disease control group(P<0.05).It is indicated that long-term aerobic exercise intervention can strengthen the proliferation,migration and differentiation of neurons during adult hippocampal neurogenesis and significantly increase the number of neuronal precursor cells and new neurons in Alzheimer’s disease mice.

关 键 词：阿尔茨海默症 有氧运动 学习记忆能力 成年海马神经发生 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学] R741.05