4-甲基伞形酮类显色底物的合成及在微生物检测中的应用研究  

SYNTHESIS OF of 4-METHYLUMBELLIFERONE CHROMOGENIC SUBSTRATE AND ITS APPLICATION IN MICROBIAL DETECTION

作　　者：尹伟 胡琪[2] 李新章 周清浩 YIN Wei;HU Qi;LI Xin-zhang;ZHOU Qin-hao(School of Bioengineering,Hefei Vocational and Technical College,Hefei,Anhui 238000,China;Chemical Composition Extraction,Separation and Identification Center of Nantong Rui feng New Material Technology Co.,Ltd.,Nantong,Jiangsu 226000,China;Department of Polymer Science and Engineering,University of Science and Technology of China,Chinese Academy of Sciences Key Laboratory of MaterialChemistry,Hefei,Anhui 230026,China)

机构地区：[1]合肥职业技术学院生物工程学院,安徽合肥238000 [2]南通睿沣新材料技术有限公司化学成分分析中心,江苏南通226000 [3]中国科学技术大学高分子科学与工程系中科院软物质化学重点实验室,安徽合肥230026

出　　处：《井冈山大学学报（自然科学版）》2023年第4期24-29,共6页Journal of Jinggangshan University (Natural Science)

基　　金：国家自然科学基金项目(21875234);安徽省高校自然科学研究项目(KJ2021ZD0158,KJ2021ZD0159);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2022124);安徽省教育厅质量工程项目(2020zyk33)。

摘　　要：以简单原料葡醛内酯,经过3步反应,对伞形酮类显色底物4-甲基-7-氧香豆素-β-D-葡萄糖苷酸进行了合成,目标产物中涉及的各步中间体,均通过核磁(^(1)H-NMR、^(13)C-NMR)等方法进行了分析表征,确定了合成工艺的可靠性。同时,通过病原微生物的荧光显色实验显示,4-甲基-7-氧香豆素-β-D-葡萄糖苷酸与大肠杆菌代谢酶结合反应后,显色效果较好,能够快速精准的检测出大肠杆菌。The umbelliferone chromogenic substrate 4-methyl-7-oxocoumarin-β-D-glucuronide was synthesized from a simple raw material glucuronide through three-step reaction,the involved intermediates in each step were analyzed and characterized by nuclear magnetic resonance(^(1)H-NMR,^(13)C-NMR)and other means,and the reliability of the synthesis process was confirmed.At the same time,the fluorescence color development experiment of pathogenic microorganisms showed that the color development effect was good after the binding reaction of 4-methyl-7-oxocoumarin-β-D-glucuronide with the metabolic enzymes of Escherichia coli,and it could quickly and accurately detect E.coli.

关 键 词：4-甲基伞形酮 显色底物 微生物 合成工艺 

分 类 号：Q623.52[生物学—生物物理学]