响应面法优化红外辅助提取紫地榆总鞣质工艺  

OPTIMIZATION OF INFRARED ASSISTED EXTRACTION PROCESS FOR TOTAL TANNIN FROM GERANIUM STRICTIPES BY RESPONSE SURFACE METHODOLOGY

作　　者：焦春香 庞博 陈俊雅 耿玲 蓝海 周萍 JIAO chun-xiang;PANG Bo;CHEN Jun-ya;GENG Ling;LAN Hai;ZHOU Ping(School of Pharmacy,Dali University,Dali,Yunnan 671000,China;Key Laboratory of Medicinal Chemistry for Natural Resource,Ministry of Education and Yunnan Province,Yunnan University,Kunming,Yunnan 65009,China;Pre-clinical College,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学药学院,云南大理671000 [2]云南大学教育部自然资源药物化学重点实验室,云南昆明650091 [3]大理大学基础医学院,云南大理671000

出　　处：《井冈山大学学报（自然科学版）》2023年第4期30-36,共7页Journal of Jinggangshan University (Natural Science)

基　　金：国家自然科学基金项目(81260512);大理大学教育部自然资源药物化学重点实验室开放基金项目(2021KF03)。

摘　　要：采用红外辅助提取紫地榆有效成分鞣质,高效液相色谱法同时检测6种鞣质成分含量,以提取时间、料液比、乙醇浓度为自变量进行3因素3水平的中心组合试验。响应面法分析得到优化提取工艺为提取1次,乙醇浓度50%,提取时间14 min,料液比1:150 g/mL,红外灯275 W,灯距1 cm。经该工艺提取,8批药材平均鞣质总含量为79.06 mg/g,与预测值80.10 mg/g相一致(P>0.05)。优化的红外辅助提取工艺稳定、可靠,为紫地榆总鞣质的有效提取提供理论依据。To optimize the extraction process for total tannin from Geranium strictipes(T-Gs),infrared assisted extraction was employed and coupled with HPLC for simultaneous determination of six compounds of T-Gs.Three-factor,three-level Box-Behnken design was used to optimize some process parameters.The most critical factors affecting the extraction of T-Gs were extraction time,material-to-liquid ratio,ethanol concentration.The optimal infrared extraction conditions for T-Gs were as following:extraction time 14 min,material-to-liquid ratio 1:150 g/mL,ethanol concentration 50%,infrared lamp height from round bottom flask 1 cm,infrared lamp power 275 W,extraction times 1 time.Under the optimal conditions,the average extraction yield of total tannin from 8 batches of medicinal materials was 79.06 mg/g,which was consistent with the theoretical extraction yield of 80.10 mg/g(P>0.05).The optimized infrared assisted extraction process for T-Gs is stable and reliable,which provided a theoretical basis for the effective extraction of T-Gs.

关 键 词：紫地榆 提取工艺 响应面法 鞣质 红外辅助提取 

分 类 号：O657.72[理学—分析化学]